Engineering cooperativity in biomotor-protein assemblies

被引：80

作者：

Diehl, MR

Zhang, KC

Lee, HJ

Tirrell, DA

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] CALTECH, Dept Appl Phys, Pasadena, CA 91125 USA

来源：

SCIENCE | 2006年 / 311卷 / 5766期

关键词：

D O I：

10.1126/science.1122125

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A biosynthetic approach was developed to control and probe cooperativity in multiunit biomotor assemblies by linking molecular motors to artificial protein scaffolds. This approach provides precise control over spatial and elastic coupling between motors. Cooperative interactions between monomeric kinesin-1 motors attached to protein scaffolds enhance hydrolysis activity and microtubule gliding velocity. However, these interactions are not influenced by changes in the elastic properties of the scaffold, distinguishing multimotor transport from that powered by unorganized monomeric motors. These results highlight the role of supramolecular architecture in determining mechanisms of collective transport.

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页码：1468 / 1471

页数：4

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