Puerarin prevents epithelial tight junction dysfunction induced by ethanol in Caco-2 cell model

被引：19

作者：

Che, Si-yan ^{[1
]}

Yuan, Jin-wen ^{[1
]}

Zhang, Li ^{[1
]}

Ruan, Zheng ^{[1
]}

Sun, Xiao-ming ^{[1
]}

Lu, Hui ^{[1
]}

机构：

[1] Nanchang Univ, Institude Nutr & Sch Food Sci, State Key Lab Food Sci & Technol, Nanchang Key Lab Fruits & Vegetables Nutr & Proc, Nanchang 330047, Jiangxi, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2020年 / 73卷

基金：

国家重点研发计划;

关键词：

Puerarin; Intestinal barrier; Tight junction; Paracellular permeability; Ethanol damage; INTESTINAL BARRIER FUNCTION; ALPHA-INDUCED LOSS; DIETARY POLYPHENOLS; ALCOHOL; EXPRESSION; INJURY; PERMEABILITY; INFLAMMATION; INTEGRITY; EXTRACT;

D O I：

10.1016/j.jff.2020.104079

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Increasing functional food ingredients such as flavonoids have been confirmed to exhibit significant effects on improving the function of intestinal barrier. Puerarin, an isoflavone extracted from Pueraria, was reported to play an active role in many diseases. Our previous research found that puerarin can improve enteritis by increasing mucin secretion and increase intestinal tight connectivity in mice. In this study, Caco-2 cell model was utilized to investigate the protective effects of puerarin on ethanol-induced intestinal tight junction injury. Results exhibited that puerarin could protect the integrity of the Caco-2 monolayer and inhibit the down-regulation of tight junction proteins (Z0-1, Claudin-1 and Occludin) expression induced by ethanol. Moreover, the puerarin administration exerted antagonistic function in regulating the alterations of tight junction related signalling pathways (NF-kappa B, ERK1 and ERK2) induced by ethanol, which may pave the way for puerarin might function as a candidate supplement to enhance intestinal barrier function.

引用

页数：9

共 48 条

[1]

Contrasting effects of ERK on tight junction integrity in differentiated and under-differentiated Caco-2 cell monolayers [J].