Hin4II, a new prototype restriction endonuclease from Haemophilus influenzae RFL4:: Discovery, cloning and expression in Escherichia coli

被引：8

作者：

Azarinskas, Audrius

Maneliene, Zita

Jakubauskas, Arturas

机构：

[1] Inst Biotechnol, LT-02241 Vilnius, Lithuania

[2] Fermentas UAB, LT-02241 Vilnius, Lithuania

来源：

JOURNAL OF BIOTECHNOLOGY | 2006年 / 123卷 / 03期

关键词：

Hin4II; restriction endonuclease; subtype IIS; Haemophilus influenzae; HNH nuclease family; restriction-modification;

D O I：

10.1016/j.jbiotec.2005.12.016

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genes encoding restriction-modification system of unknown specificity Hin4II from Haemophilus influenzae RFL4 were cloned in Escherichia coli and sequenced. The Hin4II system comprises three tandemly arranged genes coding for m6A DNA methyltransferase, m5C DNA methyltransferase and restriction endonuclease, respectively. Restriction endonuclease was expressed in E. coli and purified to apparent homogeneity. The DNA recognition sequence and cleavage positions were determined. R.Hin4II recognizes the novel non-palindromic sequence 5'-CCTTC-3' and cleaves the DNA 6 and 5 nt downstream in the top and bottom strand, respectively. The new prototype restriction endonuclease Hin4II was classified as a potential candidate of HNH nuclease family after comparison against SMART database. An amino acid sequence motif 297H-X-14-N-X-8-H of Hin4II was proposed as forming a putative catalytic center. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：288 / 296

页数：9

共 27 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, SF
Madden, TL
Schaffer, AA
Zhang, JH
Zhang, Z
Miller, W
Lipman, DJ
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
[2] Evolutionary relationship of Alw261, Eco31I and Esp3I, restriction endonucleases that recognise overlapping sequences
Bitinaite, J
Mitkaite, G
Dauksaite, V
Jakubauskas, A
Timinskas, A
Vaisvila, R
Lubys, A
Janulaitis, A
[J]. MOLECULAR GENETICS AND GENOMICS, 2002, 267 (05) : 664 - 672
[3] Crystallographic and bioinformatic studies on restriction endonucleases: Inference of evolutionary relationships in the "midnight zone" of homology
Bujnicki, JM
[J]. CURRENT PROTEIN & PEPTIDE SCIENCE, 2003, 4 (05) : 327 - 337
[4] Characterization of Alol, a restriction-modification system of a new type
Cesnaviciene, E
Petrusyte, M
Kazlauskiene, R
Maneliene, Z
Timinskas, A
Lubys, A
Janulaitis, A
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2001, 314 (02) : 205 - 216
[5] NPS@:: Network Protein Sequence Analysis
Combet, C
Blanchet, C
Geourjon, C
Deléage, G
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (03) : 147 - 150
[6] Statistical modeling and analysis of the LAGLIDADG family of site-specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH family
Dalgaard, JZ
Klar, AJ
Moser, MJ
Holley, WR
Chatterjee, A
Mian, IS
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (22) : 4626 - 4638
[7] FLEISCHMANN RD, 1995, RD SCI, V269, P496
[8] AMINO-ACID SUBSTITUTION MATRICES FROM PROTEIN BLOCKS
HENIKOFF, S
HENIKOFF, JG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (22) : 10915 - 10919
[9] KONG H, 1994, J BIOL CHEM, V269, P683
[10] MnlI - The member of H-N-H subtype of Type IIS restriction endonucleases
Kriukiene, E
Lubiene, J
Lagunavicius, A
Lubys, A
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2005, 1751 (02): : 194 - 204

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