Protein depletion from blood plasma using a volatile buffer

被引：13

作者：

Sitnikov, D ^{[1
]}

Chan, D ^{[1
]}

Thibaudeau, E ^{[1
]}

Pinard, M ^{[1
]}

Hunter, JM ^{[1
]}

机构：

[1] Caprion Pharmaceut Inc, Dept Prot Anal, Montreal, PQ H4S 2C8, Canada

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 832卷 / 01期

关键词：

immunoaffinity depletion; volatile buffer; plasma profiling; proteomics; multiple affinity removal system; albumin; immunoglobulin G;

D O I：

10.1016/j.jchromb.2005.12.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Removal of high abundance proteins is widely used in sample processing for proteomics studies of blood plasma. Immunoaffinity (IA) depletion is currently the most specific method for performing this step. Historically, IA depletion matrices have been designed to be used with inorganic buffers. However, the presence of salts in depleted samples presents a particular problem, and these must be removed in order to make samples compatible with post-depletion processing. Desalting (dialysis, ultrafittration, size-exclusion, etc.) usually diminishes sample integrity due to labware associated losses. Moreover, these steps require additional labor, increasing the processing time and cost of analysis. In order to avoid these problems, we have developed an IA method using a volatile buffer that can be removed from depleted samples by lyophilization. This method allows the execution of reproducible and efficient depletion of blood plasma in a semi-automated manner. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：41 / 46

页数：6

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