Effects of nucleus pulposus on nerve root neural activity, mechanosensitivity, axonal morphology, and sodium channel expression

被引:46
作者
Chen, CY
Cavanaugh, JM
Song, Z
Takebayashi, T
Kallakuri, S
Wooley, PH
机构
[1] Wayne State Univ, Ctr Bioengn, Spine Res Lab, Detroit, MI 48202 USA
[2] Wayne State Univ, Sch Med, Dept Orthopaed Surg, Detroit, MI USA
[3] Sapporo Med Univ, Dept Orthopaed Surg, Sapporo, Hokkaido, Japan
关键词
nucleus pulposus; nerve root; neurophysiology; mechanosensitivity; sodium channel protein; axonal histology; Schmidt-Lantenman incisure;
D O I
10.1097/01.BRS.0000096675.01484.87
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Study Design. This study analyzed the effects of autografted nucleus pulposus on nerve root axon morphology, neurophysiologic function, and sodium channel expression. Objectives. To investigate the chronic effects of the epidural implantation of nucleus pulposus on nerve root morphology, neural activity, ectopic discharge, mechanosensitivity, and sodium channel expression. Summary of Background Data. It has been reported that ectopic discharges were recorded antidromically from sural nerve on compressing nucleus pulposus exposed spinal nerves. However, it is not clear what the effects of nucleus pulposus are on ectopic discharges recorded directly from the spinal nerve roots. It is also not clear what the effects of nucleus pulposus are on the threshold pressure to provoke ectopic discharges in the spinal nerves. Sodium channel content increases in remodeling axons after nerve injury, but it is not clear what the effects of nucleus pulposus are on sodium channel expression in spinal nerve. Methods. Forty- six male Sprague- Dawley rats were used, 20 in a nucleus pulposus- implanted group, 18 in a fat- implanted group, and 8 in a normal group. Fresh autografted nucleus pulposus or fat tissue was implanted into the dorsal epidural space at the L4 - L5 disc level. On the 7th, 21st, or 42nd day, neurophysiologic recordings were made to determine nerve root response to compression. Nerve roots were then harvested to determine sodium channel protein concentration and histologic changes in the nerve root. The correlations between sodium channel density and neural activity and mechanosensitivity of dorsal root were analyzed statistically. Results. Ectopic discharge rate was higher in nucleus pulposus 7- day group. Threshold pressure to evoke ectopic discharges was lower in the nucleus pulposus 7- day group, and higher in the nucleus pulposus 42- day group compared to the normal group. Sodium channel protein density increased in the nucleus pulposus 7- day and nucleus pulposus 21- day group compared to normal nerve. Sodium channel density changes were not correlated to threshold pressure. Ectopic discharge rate increased with increase of sodium channel density in the nerve roots. The number of axons with neuropathy increased in the nucleus pulposus 7- day and 21- day groups. Conclusions. Acute exposure of nerve root to nucleus pulposus resulted in increased number of axons with neuropathy, higher intensity of ectopic discharges on compression, and nerve mechanosensitization. Chronic exposure resulted in mechanical desensitization. Changes of sodium channel density were correlated to ectopic discharge rate.
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页码:17 / 25
页数:9
相关论文
共 43 条
[1]   Immunolocalization of the Na+-K+-2Cl- cotransporter in peripheral nervous tissue of vertebrates [J].
Alvarez-Leefmans, FJ ;
León-Olea, M ;
Mendoza-Sotelo, J ;
Alvarez, FJ ;
Antón, B ;
Garduño, R .
NEUROSCIENCE, 2001, 104 (02) :569-582
[2]  
BELL GR, 1996, LUMBAR SPINE, P956
[3]   ABNORMAL MAGNETIC-RESONANCE SCANS OF THE LUMBAR SPINE IN ASYMPTOMATIC SUBJECTS - A PROSPECTIVE INVESTIGATION [J].
BODEN, SD ;
DAVIS, DO ;
DINA, TS ;
PATRONAS, NJ ;
WIESEL, SW .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1990, 72A (03) :403-408
[4]  
BOUMPHERY FRS, 1987, CLIN ORTHOP RELAT R, V219, P120
[5]   SCHMIDT-LANTERMANN INCISURES OF MYELIN SHEATH OF MAUTHNER AXONS - PLACES OF LONGITUDINAL GROWTH OF MYELIN [J].
CELIO, MR .
BRAIN RESEARCH, 1976, 108 (02) :221-235
[6]   THE EFFECT OF INTRAVENOUS LIDOCAINE, TOCAINIDE, AND MEXILETINE ON SPONTANEOUSLY ACTIVE FIBERS ORIGINATING IN RAT SCIATIC NEUROMAS [J].
CHABAL, C ;
RUSSELL, LC ;
BURCHIEL, KJ .
PAIN, 1989, 38 (03) :333-338
[7]   VIBRATION-INDUCED NEUROPHYSIOLOGICAL AND ELECTRON-MICROSCOPIC CHANGES IN RAT PERIPHERAL-NERVES [J].
CHANG, KY ;
HO, ST ;
YU, HS .
OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, 1994, 51 (02) :130-135
[8]   Effects of phospholipase A(2) on lumbar nerve root structure and function [J].
Chen, CY ;
Cavanaugh, JM ;
Ozaktay, AC ;
Kallakuri, S ;
King, AI .
SPINE, 1997, 22 (10) :1057-1064
[9]   SYSTEMIC LIDOCAINE SILENCES ECTOPIC NEUROMA AND DRG DISCHARGE WITHOUT BLOCKING NERVE-CONDUCTION [J].
DEVOR, M ;
WALL, PD ;
CATALAN, N .
PAIN, 1992, 48 (02) :261-268
[10]  
DEVOR M, 1993, J NEUROSCI, V13, P1976