K-ATP channel modulators increase survival rate during coronary occlusion-reperfusion in anaesthetized rats

We investigated the effect of ATP-sensitive K+ channel (K-ATP) openers (pinacidil and cromakalim), and a K-ATP blocker (glibenclamide) on reperfusion-induced arrhythmias in pentobarbitone-anaesthetized rats. Arrhythmias were induced by reperfusion following a 6 min ligation of the left main coronary artery. Rats were pretreated with pinacidil (0.1 or 0.3 mg/kg), or cromakalim (28 or 56 mu g/kg), or glibenclamide (5 mg/kg), or vehicle. Pinacidil and cromakalim produced dose-related reductions in blood pressure. Pinacidil (0.1 mg/kg) and cromakalim (56 mu g/kg) significantly decreased the incidence of reperfusion-induced ventricular fibrillation and increased survival. Glibenclamide did not decrease ventricular fibrillation incidence, yet improved survival by increasing the possibility of recovery from ventricular fibrillation. The present study suggests that both opening and blocking K-ATP channels may increase survival during coronary occlusion and reperfusion in anaesthetized rats. (C) 1997 Elsevier Science B.V.