Fluorescence Enhancement at Docking Sites of DNA-Directed Self-Assembled Nanoantennas

被引：571

作者：

Acuna, G. P. ^{[1
]}

Moeller, F. M. ^{[1
]}

Holzmeister, P. ^{[1
]}

Beater, S. ^{[1
]}

Lalkens, B. ^{[1
]}

Tinnefeld, P. ^{[1
]}

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Phys & Theoret Chem, NanoBioSci Grp, D-38106 Braunschweig, Germany

来源：

SCIENCE | 2012年 / 338卷 / 6106期

基金：

欧洲研究理事会;

关键词：

SINGLE-MOLECULE; NANOSCALE; EMISSION; ORIGAMI; LIGHT; MICROSCOPY; RESOLUTION; ANTENNAS; BIOLOGY; SURFACE;

D O I：

10.1126/science.1228638

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We introduce self-assembled nanoantennas to enhance the fluorescence intensity in a plasmonic hotspot of zeptoliter volume. The nanoantennas are prepared by attaching one or two gold nanoparticles (NPs) to DNA origami structures, which also incorporated docking sites for a single fluorescent dye next to one NP or in the gap between two NPs. We measured the dependence of the fluorescence enhancement on NP size and number and compare it to numerical simulations. A maximum of 117-fold fluorescence enhancement was obtained for a dye molecule positioned in the 23-nanometer gap between 100-nanometer gold NPs. Direct visualization of the binding and unbinding of short DNA strands, as well as the conformational dynamics of a DNA Holliday junction in the hotspot of the nanoantenna, show the compatibility with single-molecule assays.

引用

页码：506 / 510

页数：5

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