Assembly of the PtdIns 4-kinase Stt4 complex at the plasma membrane requires Ypp1 and Efr3

被引:119
作者
Baird, Dan [1 ,2 ]
Stefan, Chris [1 ,2 ]
Audhya, Anjon [3 ]
Weys, Sabine [1 ,2 ]
Emr, Scott D. [1 ,2 ]
机构
[1] Cornell Univ, Weill Inst Cell & Mol Biol, Ithaca, NY 14853 USA
[2] Cornell Univ, Dept Genet & Mol Biol, Ithaca, NY 14853 USA
[3] Univ Wisconsin, Dept Biomol Chem, Madison, WI 53706 USA
关键词
D O I
10.1083/jcb.200804003
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The phosphoinositide phosphatidylinositol 4-phosphate (PtdIns4P) is an essential signaling lipid that regulates secretion and polarization of the actin cytoskeleton. In Saccharomyces cerevisiae, the PtdIns 4-kinase Stt4 catalyzes the synthesis of PtdIns4P at the plasma membrane (PM). In this paper, we identify and characterize two novel regulatory components of the Stt4 kinase complex, Ypp1 and Efr3. The essential gene YPP1 encodes a conserved protein that colocalizes with Stt4 at cortical punctate structures and regulates the stability of this lipid kinase. Accordingly, Ypp1 interacts with distinct regions on Stt4 that are necessary for the assembly and recruitment of multiple copies of the kinase into phosphoinositide kinase (PIK) patches. We identify the membrane protein Efr3 as an additional component of Stt4 PIK patches. Efr3 is essential for assembly of both Ypp1 and Stt4 at PIK patches. We conclude that Ypp1 and Efr3 are required for the formation and architecture of Stt4 PIK patches and ultimately PM-based PtdIns4P signaling.
引用
收藏
页码:1061 / 1074
页数:14
相关论文
共 59 条
[1]   Involvement of the late secretory pathway in actin regulation and mRNA transport in yeast [J].
Aronov, S ;
Gerst, JE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (35) :36962-36971
[2]   Distinct roles for the yeast phosphatidylinositol 4-kinases, Stt4p and Pik1p, in secretion, cell growth, and organelle membrane dynamics [J].
Audhya, A ;
Foti, M ;
Emr, SD .
MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (08) :2673-2689
[3]   Stt4 PI 4-kinase localizes to the plasma membrane and functions in the Pkc1-mediated MAP kinase cascade [J].
Audhya, A ;
Emr, SD .
DEVELOPMENTAL CELL, 2002, 2 (05) :593-605
[4]  
Blatch GL, 1999, BIOESSAYS, V21, P932, DOI 10.1002/(SICI)1521-1878(199911)21:11<932::AID-BIES5>3.3.CO
[5]  
2-E
[6]   Gene recruitment of the activated INO1 locus to the nuclear membrane [J].
Brickner, JH ;
Walter, P .
PLOS BIOLOGY, 2004, 2 (11) :1843-1853
[7]   TPR proteins: the versatile helix [J].
D'Andrea, LD ;
Regan, L .
TRENDS IN BIOCHEMICAL SCIENCES, 2003, 28 (12) :655-662
[8]   Phosphoinositides as regulators in membrane traffic [J].
DeCamilli, P ;
Emr, SD ;
McPherson, PS ;
Novick, P .
SCIENCE, 1996, 271 (5255) :1533-1539
[9]   Phosphoinositides in cell regulation and membrane dynamics [J].
Di Paolo, Gilbert ;
De Camilli, Pietro .
NATURE, 2006, 443 (7112) :651-657
[10]  
Faulkner DL, 1998, DEV GENET, V23, P264, DOI 10.1002/(SICI)1520-6408(1998)23:4<264::AID-DVG2>3.0.CO