The transplantation of human fetal neuroretinal cells in advanced retinitis pigmentosa patients: Results of a long-term safety study

被引:68
作者
Das, T
del Cerro, M
Jalali, S
Rao, VS
Gullapalli, VK
Little, C
Loreto, DAD
Sharma, S
Sreedharan, A
del Cerro, C
Rao, GN
机构
[1] LV Prasad Eye Inst, Hyderabad 500034, Andhra Pradesh, India
[2] Univ Rochester, Sch Med, Rochester, NY 14642 USA
[3] Dept Ophthalmol, Rochester, NY 14642 USA
关键词
fetal cells; human; photoreceptors; retina; retinal degeneration; transplantation; retinitis pigmentosa;
D O I
10.1006/exnr.1998.6992
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The purpose of this study was to determine the long-term safety of transplanting human fetal neuroretinal cells (14 to 18 week gestational age) into a series of patients with advanced retinitis pigmentosa (RP). After obtaining informed consent, both hosts and mothers of donors were screened for transmissible diseases. Pre- and postoperative clinical exams, visual acuity, electroretinograms, and fluorescein angiograms were performed and visual field testing was attempted in each case. Surgically, an anterior approach through pars plana ciliaris was used. A retinotomy was performed in the paramacular area and a two-function cannula was introduced into the subretinal space to deliver a suspension of donor cells. The cell suspension carried approximately 4000 cells/mu l; the volume injected did not exceed 150 mu l. The patients were examined for periods ranging from 12 to 40 months posttransplantation. To date, no evidence of inflammation, infection, or overt rejection of the graft was noted in the host eye, neither was any change observed in the contralateral, unoperated eye. In conclusion, neuroretinal cells were injected into the subretinal space of 14 patients with advanced RP with no clinical appearance of detrimental effects at the time of surgery or up to 40 months postinjection except in 1 patient who developed retinal detachment. This sets the stage for a phase II clinical trial to determine the possible beneficial effects of this procedure in patients blinded by degenerative retinal disease. (C) 1999 Academic Press.
引用
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页码:58 / 68
页数:11
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