Preparation and Electrochemical Behavior of L-Glutamate Electrochemical Biosensor

被引：256

作者：

Deng, Yan ^{[1
,2
]}

Wang, Wei ^{[1
]}

Zhang, Liming ^{[1
,2
]}

Lu, Zhuoxuan ^{[1
,2
]}

Li, Song ^{[1
]}

Xu, Lijian ^{[1
]}

机构：

[1] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China

[2] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2013年 / 9卷 / 02期

关键词：

L-Glutamate; Biosensor; Electrochemical; Nano-Porous Pseudo Carbon Paste Electrode; CARBON NANOTUBES; MAGNETIC NANOPARTICLES; MASS-SPECTROMETRY; ACID; OXIDASE; SYSTEM; CANCER; ZNO;

D O I：

10.1166/jbn.2013.1487

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

Using the nano-porous pseudo carbon paste electrode (Nano-PPCPE) as the working electrode, mixing L-glutamate oxidase (L-GLOD), catalase (Cat) and bovine serum albumin (BSA) with phosphate buffer (PB, pH = 7.4), followed by cross-linking with glutaraldehyde, a novel L-glutamate electrochemical biosensor was successfully formed. It was demonstrated that the modified nano-PPCPE exhibits a high selectivity and sensitivity in comparison with the modified CPE. The L-glutamate biosensor showed a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M.

引用

收藏

页码：318 / 321

页数：4

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