Hypoxic culture induces expression of sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells

被引:132
作者
Yin, J
Hashimoto, A
Izawa, M
Miyazaki, K
Chen, GY
Takematsu, H
Kozutsumi, Y
Suzuki, A
Furuhata, K
Cheng, FL
Lin, CH
Sato, C
Kitajima, K
Kannagi, R
机构
[1] Aichi Canc Ctr, Dept Mol Pathol, Res Inst, Chikusa Ku, Nagoya, Aichi 4648681, Japan
[2] Nagoya Univ, Grad Sch Bioagr Sci, Dept Bioengn Sci, Nagoya, Aichi, Japan
[3] CREST, Japan Sci & Technol Agcy, Kawaguchi, Japan
[4] Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan
[5] Kitasato Univ, Sch Pharmaceut Sci, Tokyo 108, Japan
关键词
D O I
10.1158/0008-5472.CAN-05-2615
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Tumor hypoxia figures heavily in malignant progression by altering the intracellular glucose metabolism and inducing angiogenic factor production, thus, selecting and expanding more aggressive cancer cell clones. Little is known, however, regarding hypoxia-induced antigenic changes in cancers. We investigated the expression of N-glycolyl sialic acid (NeuGc)-G(M2), a cancer-associated ganglioside containing non-human sialic acid, NeuGc, in human cancers. Cancer tissues prepared from patients with colon cancers frequently expressed NeuGc-G(M2), whereas it was virtually absent in nonmalignant colonic epithelia. Studies on cultured cancer cells indicated that the non-human sialic acid was incorporated from culture medium. Hypoxic culture markedly induced mRNA for a sialic acid transporter, sialin, and this accompanied enhanced incorporation of NeuGc as well as N-acetyl sialic acid. Transfection of cells with sialin gene conferred accelerated sialic acid transport and induced cell surface expression of NeuGc-G(M2). We propose that the preferential expression of NeuGc-G(M2) in cancers is closely associated with tumor hypoxia. Hypoxic culture of tumor cells induces expression of the sialic acid transporter, and enhances the incorporation of non-human sialic acid from the external milieu. A consequence of this is the acquisition of cancer-associated cell surface gangliosides, typically G(M2), containing non-human sialic acid (NeuGc), which is not endogenously synthesized through CMP-N-acetyl sialic acid hydroxylase because humans lack the gene for the synthetic enzyme. As hypoxia is associated with diminished response to radiotherapy and chemotherapy, NeuGc-G(M2) is a potential therapeutic target for hypoxic cancer cells.
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收藏
页码:2937 / 2945
页数:9
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