Temperature gradient interaction chromatography of low molecular weight polystyrene

Separation of low molecular weight polystyrene (PS) by temperature gradient interaction chromatography (TGIC) is reported, As in the separation of high molecular weight PS, temperature is an efficient variable to control the retention of low molecular weight PS in the reversed phase HPLC separation. However, a right choice of the eluent, typically a marginal solvent for the polymeric solute of interest, is crucial for the temperature to play an effective role in the retention control. For an example, PS oligomers were well separated by TGIC under the isocratic elution condition of C18 bonded silica and methanol as the stationary and the mobile phase, respectively. (C) 1999 Elsevier Science Ltd, All rights reserved.