A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana

被引：128

作者：

Kamada-Nobusada, Tomoe ^{[1
,3
]}

Hayashi, Makoto ^{[1
,2
]}

Fukazawa, Mitsue ^{[1
]}

Sakakibara, Hitoshi ^{[3
]}

Nishimura, Mikio ^{[1
,2
]}

机构：

[1] Natl Inst Basic Biol, Dept Cell Biol, Div Cell Mech, Okazaki, Aichi 4448585, Japan

[2] Grad Univ Adv Studies, Sch Life Sci, Dept Mol Biomech, Okazaki, Aichi 4448585, Japan

[3] RIKEN, Plant Sci Ctr, Yokohama, Kanagawa 2300045, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2008年 / 49卷 / 09期

基金：

日本学术振兴会;

关键词：

Arabidopsis; peroxisome; polyamine; polyamine oxidase; spermidine; spermine;

D O I：

10.1093/pcp/pcn114

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We characterized three Arabidopsis polyamine oxidase genes, AtPAO2, AtPAO3 and AtPAO4. Transient expression of these genes as monomeric red fluorescent protein fusion proteins in Arabidopsis root cells revealed that all are peroxisomal proteins. Quantitative analysis of their transcripts in various organs suggested that AtPAO4 is the major isoform in root peroxisomes. Analysis of recombinant AtPAO4 protein indicated that it is a flavoprotein that catalyzed the oxidative conversion of spermine to spermidine. AtPAO4-deficient mutants established by using T-DNA insertion and RNA interference techniques had markedly increased spermine and decreased spermidine levels in the roots. These results suggest that AtPAO4 is a root peroxisomal polyamine oxidase that participates in polyamine catabolism. Microarray analysis showed that AtPAO4 deficiency induced alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis.

引用

页码：1272 / 1282

页数：11

共 45 条

[1] Abscisic acid modulates polyamine metabolism under water stress in Arabidopsis thaliana [J].

Alcazar, Ruben ;

Cuevas, Juan C. ;

Patron, M. ;

Altabella, Teresa ;

Tiburcio, Antonio F. .

PHYSIOLOGIA PLANTARUM, 2006, 128 (03) :448-455

[2] Involvement of polyamines in plant response to abiotic stress [J].

Alcazar, Ruben ;

Marco, Francisco ;

Cuevas, Juan C. ;

Patron, Macarena ;

Ferrando, Alejandro ;

Carrasco, Pedro ;

Tiburcio, Antonio F. ;

Altabella, Teresa .

BIOTECHNOLOGY LETTERS, 2006, 28 (23) :1867-1876

[3] MAIZE POLYAMINE OXIDASE - ANTIBODY-PRODUCTION AND ULTRASOUND LOCALIZATION [J].

ANGELINI, R ;

FEDERICO, R ;

BONFANTE, P .

JOURNAL OF PLANT PHYSIOLOGY, 1995, 145 (5-6) :686-692

[4]

[Anonymous], 1991, BIOCH PHYSL POLYAMIN

[5]

BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194

[6] MICROBODIES IN HIGHER-PLANTS [J].

BEEVERS, H .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1979, 30 :159-193

[7] Flavonoid accumulation in Arabidopsis repressed in lignin synthesis affects auxin transport and plant growth [J].

Besseau, Sebastien ;

Hoffmann, Laurent ;

Geoffroy, Pierrette ;

Lapierre, Catherine ;

Pollet, Brigitte ;

Legrand, Michel .

PLANT CELL, 2007, 19 (01) :148-162

[8] Polyamines and environmental challenges: recent development [J].

Bouchereau, A ;

Aziz, A ;

Larher, F ;

Martin-Tanguy, J .

PLANT SCIENCE, 1999, 140 (02) :103-125

[9] Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress [J].

Capell, T ;

Bassie, L ;

Christou, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (26) :9909-9914

[10] Isolation and characterization of three polyamine oxidase genes from Zea mays [J].

Cervelli, M ;

Tavladoraki, P ;

Di Agostino, S ;

Angelini, R ;

Federico, R ;

Mariottini, P .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2000, 38 (09) :667-677

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