Packaging of actin into Ebola virus VLPs

被引：39

作者：

Han, Ziying ^{[1
]}

Harty, Ronald N. ^{[1
]}

机构：

[1] Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA 19104 USA

来源：

VIROLOGY JOURNAL | 2005年 / 2卷 / 1期

关键词：

West Nile Virus; Newcastle Disease Virus; Equine Infectious Anemia Virus; Cellular Actin; Murine Mammary Tumor Virus;

D O I：

10.1186/1743-422X-2-92

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The actin cytoskeleton has been implicated in playing an important role assembly and budding of several RNA virus families including retroviruses and paramyxoviruses. In this report, we sought to determine whether actin is incorporated into Ebola VLPs, and thus may play a role in assembly and/or budding of Ebola virus. Our results indicated that actin and Ebola virus VP40 strongly co-localized in transfected cells as determined by confocal microscopy. In addition, actin was packaged into budding VP40 VLPs as determined by a functional budding assay and protease protection assay. Co-expression of a membrane-anchored form of Ebola virus GP enhanced the release of both VP40 and actin in VLPs. Lastly, disruption of the actin cytoskeleton with latrunculin-A suggests that actin may play a functional role in budding of VP40/GP VLPs. These data suggest that VP40 may interact with cellular actin, and that actin may play a role in assembly and/or budding of Ebola VLPs.

引用

页码：1 / 5

页数：5

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