Exposure to N-nitroso compounds in a population of high liver cancer regions in Thailand:: Volatile nitrosamine (VNA) levels in Thai food

The recent case-control studies in Thailand indicate that a high incidence of liver cancer in Thailand has not been associated with common risk factors such as hepatitis B infection, aflatoxin intake and alcohol consumption. While the infestation by the liver fluke Opisthorchis viverrini (OV) accounted for the high risk in north-east Thailand, there a cis no such exposure: in the other regions of the country what: the incidence of liver cancer is also high. Case-control studies suggest that exposure to exogenous and possibly endogenous nitrosamines in food or tobacco in betel nut and cigarettes mag; play a role in the development of hepatocellular carcinoma (HCC), while OV infestation and chemical interaction of nitrosamines may also be aetiological factors in the development of cholangiocarcinoma (CCA) Over 1800 samples of Fresh and preserved food were systematically collected and tested between 1988 and 1996. All the food items identified by anthropological studies to be consumed frequently in four major regions of Thailand were analysed for volatile nitrosamines using gas chromatography combined with a thermal energy analyser. Relatively high levels of N-nitrosodimethylamine (NDPA). N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine (NPYR) a-srs detected in fermented fish ("Pla-salid"). NDMA was also detected at levels ranging from trace amounts to 66.5 mu g/kg in several salted and dried fish ("Larb-pla" and "Pla-siu"). NDMA and NPYR were frequently detected in several vegetables, particularly fermented beans ("Tau-chiau") at levels ranging between 1 and 95.1 mu g/kg and 0 146 mu g/kg, respectively. The possible role of nitrosamines in Thai food in the aetiology of liver cancer (HCC, CCA) is discussed. 1999 Elsevier Science Ltd. All rights reserved.