Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum

被引：20

作者：

DeBarber, Andrea E. ^{[1
]}

Geraci, Travis ^{[1
]}

Colasurdo, Vincent P. ^{[1
]}

Hackenmueller, Sarah A. ^{[1
]}

Scanlan, Thomas S. ^{[1
]}

机构：

[1] Oregon Hlth & Sci Univ, Dept Physiol & Pharmacol, Portland, OR 97239 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1210卷 / 01期

关键词：

3-Iodothyronamine; LC-MS/MS; Serum;

D O I：

10.1016/j.chroma.2008.09.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity. (c) 2008 Elsevier B.V. All rights reserved.

引用

收藏

页码：55 / 59

页数：5

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