Cryopreserved chronic lymphocytic leukemia cells analyzed by multicolor fluorescence in situ hybridization after optimized mitogen stimulation

被引：4

作者：

Karhu, R

Vilpo, L

Isola, J ^{[1
]}

Knuutila, S

Vilpo, J

机构：

[1] Univ Tampere, Inst Med Technol, Canc Genet Lab, Tampere 33014, Finland

[2] Tampere Univ Hosp, Tampere, Finland

[3] Univ Tampere, Sch Med, Leukemia Res Lab, Dept Clin Chem, FIN-33101 Tampere, Finland

[4] Univ Helsinki, Dept Med Genet, Haartman Inst, Helsinki, Finland

[5] Univ Helsinki, Cent Hosp, Helsinki, Finland

来源：

GENES CHROMOSOMES & CANCER | 2002年 / 34卷 / 03期

关键词：

D O I：

10.1002/gcc.10074

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We investigated the utility of multicolor in situ fluorescence hybridization (mFISH) on cryopreserved blood cells from I I chronic lymphocytic leukemia (CLL) patients. The results demonstrate that an individually chosen optimized mitogen combination induces proliferation of neoplastic B-cells after cryopreservation. Abnormal cells were detected in eight samples by mFISH, and, in six samples, the abnormality could be verified by comparative genomic hybridization or interphase FISH. In addition to typical CLL abnormalities, such as del(11q) or +12, several balanced translocations and single-cell abnormalities were found. Thus, mFISH can reveal new prognostically relevant chromosome aberrations in CLL. (C) 2002 Wiley-Liss, Inc.

引用

页码：345 / 348

页数：4

共 11 条

[1] Genomic aberrations and survival in chronic lymphocytic leukemia. [J].