Analysis of flavonol glycoside isomers from leaves of Maytenus ilicifolia by offline and online high performance liquid chromatography-electrospray mass spectrometry

被引：40

作者：

de Souza, Lauro M. ^{[1
]}

Cipriani, Thales R. ^{[1
]}

Serrato, Rodrigo V. ^{[1
]}

da Costa, Denise E. ^{[1
]}

Iacomini, Marcello ^{[1
]}

Gorin, Philip A. J. ^{[1
]}

Sassaki, Guilherme L. ^{[1
]}

机构：

[1] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1207卷 / 1-2期

关键词：

Liquid chromatography-mass spectrometry; Maytenus ilicifolia; Flavonol glycosides isomers; Kaempferol; Quercetin; Radical aglycone;

D O I：

10.1016/j.chroma.2008.08.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flavonol glycosides present in leaves of Maytenus ilicifolia, were examined after fractionation on silica-gel column. Flavonol mono-, di-, tri-, and tetraglycosides, containing kaempferol, quercetin or myricetin were identified by offline electrospray mass spectrometry. Increasing the cone energy induced to adducts variation, from H+ to Na+. Protonated ions were characteristically fragmented by sequentially removing the monosaccharide residues, whereas in the sodiated ions, the aglycone was firstly removed. Online high performance liquid chromatography-mass spectrometry, with simple gradients of water, acetonitrile and acetic acid indicated the presence of several isomers, which were further identified by gas chromatography-mass spectrometry as containing galactose or glucose. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：101 / 109

页数：9

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