Discovering new natural products directly from crude extracts by HPLC-SPE-NMR:: Chinane diterpenes in Harpagophytum procumbens

被引：36

作者：

Clarkson, C

Staerk, D

Hansen, SH

Smith, PJ

Jaroszewski, JW

机构：

[1] Danish Univ Pharmaceut Sci, Dept Med Chem, DK-2100 Copenhagen, Denmark

[2] Danish Univ Pharmaceut Sci, Dept Pharmaceut & Analyt Chem, DK-2100 Copenhagen, Denmark

[3] Univ Cape Town, Groote Schuur Hosp, Dept Med, Div Pharmacol, ZA-7925 Cape Town, South Africa

来源：

JOURNAL OF NATURAL PRODUCTS | 2006年 / 69卷 / 04期

关键词：

D O I：

10.1021/np050504g

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

HPLC-SPE-NMR experiments with crude extracts of Harpagophytum procumbens allowed the rapid identification of novel, unstable chinane-type tricyclic diterpenes (1 and 2), along with numerous other constituents. Dramatic sensitivity gains achieved with this novel hyphenated technique establish a new paradigm in natural products research: rapid and rigorous structure determination of multiple extract components, including minor components, without preparative-scale isolation.

引用

页码：527 / 530

页数：4

共 33 条

[1] TRICYCLIC DITERPENES FROM THE STEM BARK OF AZADIRACHTA-INDICA [J].