Low-cost, frequency-domain, fluorescence lifetime confocal microscopy

被引：57

作者：

Booth, MJ ^{[1
]}

Wilson, T ^{[1
]}

机构：

[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

来源：

JOURNAL OF MICROSCOPY-OXFORD | 2004年 / 214卷

关键词：

confocal microscopy; fluorescence lifetime; laser diode; quadrative demodulation;

D O I：

10.1111/j.0022-2720.2004.01316.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection. This allows the rapid acquisition of fluorescence intensity and lifetime images and their display in real time. We show fluorescence lifetime images of bead objects and fluorescence lifetime images of biological specimens from a single confocal scan.

引用

页码：36 / 42

页数：7

共 16 条

[1]

[Anonymous], 1990, CONFOCAL MICROSCOPY

[2] Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single-photon counting (TCSPC) [J].