An OspC-specific monoclonal antibody passively protects mice from tick-transmitted infection by Borrelia burgdorferi B31

被引：64

作者：

Mbow, ML ^{[1
]}

Gilmore, RD

Titus, RG

机构：

[1] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Pathol, Ft Collins, CO 80523 USA

[2] Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, Publ Hlth Serv,US Dept Hlth & Human Serv, Ft Collins, CO USA

来源：

INFECTION AND IMMUNITY | 1999年 / 67卷 / 10期

关键词：

D O I：

10.1128/IAI.67.10.5470-5472.1999

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A murine monoclonal antibody directed against Borrelia burgdorferi B31 outer surface protein C (OspC) antigen was generated by a method whereby borreliae were inoculated into the mouse via the natural transmission mode of tick feeding. Passive immunization with this antibody resulted in protection of C3H/HeJ and outbred mice from a tick-transmitted challenge infection. Immunofluorescence staining of borrelia cells indicated surface exposure of the OspC epitope reactive with the monoclonal antibody.

引用

页码：5470 / 5472

页数：3

共 17 条

[1] Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice [J].

Bockenstedt, LK ;

Hodzic, E ;

Feng, SL ;

Bourrel, KW ;

deSilva, A ;

Montgomery, RR ;

Fikrig, E ;

Radolf, JD ;

Barthold, SW .

INFECTION AND IMMUNITY, 1997, 65 (11) :4661-4667

[2] Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine [J].

deSilva, AM ;

Telford, SR ;

Brunet, LR ;

Barthold, SW ;

Fikrig, E .

JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (01) :271-275

[3] ELIMINATION OF BORRELIA-BURGDORFERI FROM VECTOR TICKS FEEDING ON OSPA-IMMUNIZED MICE [J].

FIKRIG, E ;

TELFORD, SR ;

BARTHOLD, SW ;

KANTOR, FS ;

SPIELMAN, A ;

FLAVELL, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5418-5421

[4] A monoclonal antibody generated by antigen inoculation via tick bite is reactive to the Borrelia burgdorferi Rev protein, a member of the 2.9 gene family focus [J].

Gilmore, RD ;

Mbow, ML .

INFECTION AND IMMUNITY, 1998, 66 (03) :980-986

[5] Outer surface protein C (OspC), but not P39, is a protective immunogen against a tick-transmitted Borrelia burgdorferi challenge: Evidence for a conformational protective epitope in OspC [J].

Gilmore, RD ;

Kappel, KJ ;

Dolan, MC ;

Burkot, TR ;

Johnson, BJB .

INFECTION AND IMMUNITY, 1996, 64 (06) :2234-2239

[6] PROTECTIVE IMMUNITY IS INDUCED BY A BORRELIA-BURGDORFERI MUTANT THAT LACKS OSPA AND OSPB [J].

HUGHES, CAN ;

ENGSTROM, SM ;

COLEMAN, LA ;

KODNER, CB ;

JOHNSON, RC .

INFECTION AND IMMUNITY, 1993, 61 (12) :5115-5122

[7] Resistance to tick-borne spirochete challenge induced by Borrelia burgdorferi strains that differ in expression of outer surface proteins [J].

Kurtti, TJ ;

Munderloh, UG ;

Hughes, CAN ;

Engstrom, SM ;

Johnson, RC .

INFECTION AND IMMUNITY, 1996, 64 (10) :4148-4153

[8] STANDARD SYSTEM FOR INFECTING TICKS (ACARI, IXODIDAE) WITH THE LYME-DISEASE SPIROCHETE, BORRELIA-BURGDORFERI [J].

PIESMAN, J .

JOURNAL OF MEDICAL ENTOMOLOGY, 1993, 30 (01) :199-203

[9] ACTIVE IMMUNIZATION WITH PC PROTEIN OF BORRELIA-BURGDORFERI PROTECTS GERBILS AGAINST BORRELIA-BURGDORFERI INFECTION [J].

PREACMURSIC, V ;

WILSKE, B ;

PATSOURIS, E ;

JAURIS, S ;

WILL, G ;

SOUTSCHEK, E ;

RAINHARDT, S ;

LEHNERT, G ;

KLOCKMANN, U ;

MEHRAEIN, P .

INFECTION, 1992, 20 (06) :342-349

[10] PROTECTION OF C3H/HEN MICE FROM CHALLENGE WITH BORRELIA-BURGDORFERI THROUGH ACTIVE IMMUNIZATION WITH OSPA, OSPB, OR OSPC, BUT NOT WITH OSPD OR THE 83-KILODALTON ANTIGEN [J].

PROBERT, WS ;

LEFEBVRE, RB .

INFECTION AND IMMUNITY, 1994, 62 (05) :1920-1926

← 1 2 →