Effect of polypeptide 2B1 on condition of dampness pattern in rats in terms of Traditional Chinese Medicine

被引：6

作者：

Yang, Zhangbin ^{[1
,2
]}

Xiang, Ting ^{[1
]}

Zhang, Shijun ^{[1
]}

Zhan, Hongrui ^{[3
]}

Chen, Zexiong ^{[1
]}

Sun, Baoguo ^{[1
]}

Chen, Xiaoling ^{[1
]}

Shi, Jingli ^{[2
]}

Ren, Bin ^{[4
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Tradit Chinese Med, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 5, Dept Tradit Chinese Med, Guangzhou 519000, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Affiliated Hosp 5, Dept Phys Med & Rehabilitat, Guangzhou 519000, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Pharm, Guangzhou 510080, Guangdong, Peoples R China

来源：

JOURNAL OF TRADITIONAL CHINESE MEDICINE | 2014年 / 34卷 / 02期

关键词：

Medicine; Chinese traditional; Viscera; Organic anion transporter polypeptide 2B1; Dampness pattern; Aristolochic acid I; REAL-TIME PCR; EXPRESSION; KIDNEY; LIVER;

D O I：

10.1016/S0254-6272(14)60081-9

中图分类号：

R [医药、卫生];

学科分类号：

100218 [急诊医学];

摘要：

OBJECTIVE: This study investigated how polypeptide 2B1 is involved in regulating and governing dampness in rat models with dampness pattern defined in terms of Traditional Chinese Medicine. METHODS: We randomly divided 48 SPF 10-week-old male Sprague-Dawley (SD) rats into a normal group, normal + Aristolochic acid I (AA-1) for 5 min group, normal + AA-I for 60 min group, dampness pattern group (DS-Group), dampness pattern + AA-I for 5 min group, and dampness pattern + AA-I for 60 min group. Groups were then treated accordingly. We took out the lung, stomach, liver, spleen, kidney, large intestine, and small intestine tissues to detect gene and protein expression of organic anion transporter polypeptide 2B1 (OATP2B1). RESULTS: Gene expression of OATP2B1 in spleen, kidney, and small intestine of rats with dampness pattern was lower than that in normal rats (P < 0.05). The gene expressions of OATP2B1 in liver, stomach, large intestine, and small intestine were lower than that in control rats at different time points after being stimulated by AA-1 (P < 0.05). CONCLUSION: There is coordination among multiple viscera in handling the condition of dampness, and the mechanism underlying the action may rely on regulating the expression of OATP2B1.

引用

页码：214 / 220

页数：7

共 20 条

[1]

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