Quantification of the adhesion free energy between bacteria and hydrophobic and hydrophilic substrata

被引:97
作者
Bayoudh, S
Othmane, A [1 ]
Bettaieb, F
Bakhrouf, A
Ben Ouada, H
Ponsonnet, L
机构
[1] Fac Med, Biophys Lab, Monastir, Tunisia
[2] Fac Sci, Lab Phys & Chim Interfaces, Monastir, Tunisia
[3] Fac Pharm Monastir, Lab Analyse & Controle Polluants Chim & Biol Envi, Monastir, Tunisia
[4] Univ Lyon 1, Ecole Cent Lyon, Ctr Genie Elect, F-69365 Lyon, France
来源
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2006年 / 26卷 / 2-3期
关键词
bacterial adhesion; contact angle; adhesion energy;
D O I
10.1016/j.msec.2005.10.045
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
In this paper, the bacterial adhesion to hydrophilic glass and hydrophobic indium tin oxide (ITO) coated glass surfaces was investigated. The aim of this work was to examine the effect of the hydrophobicity of two bacterial strains (Pseudomonas stutzeri PS, and Staphylococcus epidermidis SE) and substrata surfaces on the adhesion mechanism. The hydrophobicity of the surfaces of the bacterial strains as well as of the surfaces of the tested materials was characterized using the contact angle measurement via sessile drop technique. The water contact angle measurements of the intrinsic cell hydrophobicity showed that the PS strain is more hydrophobic (theta=40 degrees) than SE strain (theta=21 degrees). For both tested materials, this angle was 12 degrees for glass surface and 84 degrees for ITO-coated glass surface. Based on the thermodynamic approach, the Lifshitz van der Waals interaction free energy, the hydrophobic interaction free energy and the total interaction free energy between the bacteria and the substratum through the suspending medium were quantified. In order to verify the thermodynamic approach predictions of the bacterial adhesion on each substratum, adhesion tests were carried out for each bacteria/substratum combination. The results revealed that for both materials surfaces, the adhesion energy of the hydrophobic PS strain (26.1 mJ/m(2) for glass and -3.8 mJ/m(2) for ITO-coated glass) is higher than that of SE strain (31.8 mJ/m(2) for glass and 14.3 mJ/m(2) for ITO-coated glass). For both bacterial strains, the effect of the hydrophobicity property of the substratum seems more important in the initial adhesion step. However, the second step of adhesion involves the biological approach, since a discrepancy was found between physicochemical theoretical approach and adhesion tests for SE strain. (c) 2005 Elsevier B.V All rights reserved.
引用
收藏
页码:300 / 305
页数:6
相关论文
共 21 条
[1]  
ADAMSON AW, 1990, PHYSICAL CHEM SURFAC, P107
[2]   Bacterial strains isolated from different niches can exhibit different patterns of adhesion to substrata [J].
Bakker, DP ;
Postmus, BR ;
Busscher, HJ ;
van der Mei, HC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (06) :3758-3760
[3]   A COMPARISON OF THERMODYNAMIC APPROACHES TO PREDICT THE ADHESION OF DAIRY MICROORGANISMS TO SOLID SUBSTRATA [J].
BELLONFONTAINE, MN ;
MOZES, N ;
VANDERMEI, HC ;
SJOLLEMA, J ;
CERF, O ;
ROUXHET, PG ;
BUSSCHER, HJ .
CELL BIOPHYSICS, 1990, 17 (01) :93-106
[4]   Adhesion of Lactococcus lactis to model substrata:: direct study of the interface [J].
Boonaert, CJP ;
Dufrêne, YF ;
Derclaye, SR ;
Rouxhet, PG .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2001, 22 (03) :171-182
[5]   Bacterial adhesion to surface hydrophilic and hydrophobic contact lenses [J].
Bruinsma, GM ;
van der Mei, HC ;
Busscher, HJ .
BIOMATERIALS, 2001, 22 (24) :3217-3224
[6]   Thermodynamic analysis of growth temperature dependence in the adhesion of Candida parapsilosis to polystyrene [J].
Gallardo-Moreno, AM ;
González-Martín, ML ;
Pérez-Giraldo, C ;
Garduño, E ;
Bruque, JM ;
Gómez-García, AC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (05) :2610-2613
[7]   Serum as a factor influencing adhesion of Enterococcus faecalis to glass and silicone [J].
Gallardo-Moreno, AM ;
González-Martín, ML ;
Pérez-Giraldo, C ;
Bruque, JM ;
Gómez-García, AC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (11) :5784-5787
[8]   Analysis of bacterial detachment from substratum surfaces by the passage of air-liquid interfaces [J].
Gómez-Suárez, C ;
Busscher, HJ ;
van der Mei, HC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (06) :2531-2537
[9]   Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion [J].
Hallab, NJ ;
Bundy, KJ ;
O'Connor, K ;
Moses, RL ;
Jacobs, JJ .
TISSUE ENGINEERING, 2001, 7 (01) :55-71
[10]  
Israelachvili J.N., 1992, INTERMOLECULAR SURFA, Vsecond, P16