Expression and self-assembly of Norwalk virus capsid protein from Venezuelan equine encephalitis virus replicons

被引：69

作者：

Baric, RS ^{[1
]}

Yount, B

Lindesmith, L

Harrington, PR

Greene, SR

Tseng, FC

Davis, N

Johnston, RE

Klapper, DG

Moe, CL

机构：

[1] Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Sch Med, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA

来源：

JOURNAL OF VIROLOGY | 2002年 / 76卷 / 06期

关键词：

D O I：

10.1128/JVI.76.6.3023-3030.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis virus replicon particles (VRP-WI). VRP-NV1 infection resulted in large numbers of recombinant NV-like particles that were primarily cell associated and were indistinguishable from NV particles produced from baculoviruses. Mutations located in the N-terminal and PI domains of the W capsid protein ablated capsid self-assembly in mammalian cells.

引用

页码：3023 / 3030

页数：8

共 50 条

[1] Noncytopathic Sindbis virus RNA vectors for heterologous gene expression [J].

Agapov, EV ;

Frolov, I ;

Lindenbach, BD ;

Pragai, BM ;

Schlesinger, S ;

Rice, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (22) :12989-12994

[2] Oral immunization with recombinant Norwalk virus-like particles induces a systemic and mucosal immune response in mice [J].

Ball, JM ;

Hardy, ME ;

Atmar, RL ;

Conner, ME ;

Estes, MK .

JOURNAL OF VIROLOGY, 1998, 72 (02) :1345-1353

[3] Recombinant Norwalk virus-like particles given orally to volunteers: Phase I study [J].

Ball, JM ;

Graham, DY ;

Opekun, AR ;

Gilger, MA ;

Guerrero, RA ;

Estes, MK .

GASTROENTEROLOGY, 1999, 117 (01) :40-48

[4] Dendritic cells and the control of immunity [J].

Banchereau, J ;

Steinman, RM .

NATURE, 1998, 392 (6673) :245-252

[5] Persistent infection promotes cross-species transmissibility of mouse hepatitis virus [J].

Baric, RS ;

Sullivan, E ;

Hensley, L ;

Yount, B ;

Chen, W .

JOURNAL OF VIROLOGY, 1999, 73 (01) :638-649

[6] Humoral, mucosal, and cellular immunity in response to a human immunodeficiency virus type 1 immunogen expressed by a Venezuelan equine encephalitis virus vaccine vector [J].

Caley, IJ ;

Betts, MR ;

Irlbeck, DM ;

Davis, NL ;

Swanstrom, R ;

Frelinger, JA ;

Johnston, RE .

JOURNAL OF VIROLOGY, 1997, 71 (04) :3031-3038

[7] Mucosal immunity induced by parenteral immunization with a live attenuated Venezuelan equine encephalitis virus vaccine candidate [J].

Charles, PC ;

Brown, KW ;

Davis, NL ;

Hart, MK ;

Johnston, RE .

VIROLOGY, 1997, 228 (02) :153-160

[8] Vaccination of macaques against pathogenic simian immunodeficiency virus with Venezuelan equine encephalitis virus replicon particles [J].

Davis, NL ;

Caley, IJ ;

Brown, KW ;

Betts, MR ;

Irlbeck, DM ;

McGrath, KM ;

Connell, MJ ;

Montefiori, DC ;

Frelinger, JA ;

Swanstrom, R ;

Johnson, PR ;

Johnston, RE .

JOURNAL OF VIROLOGY, 2000, 74 (01) :371-378

[9] A viral vaccine vector that expresses foreign genes in lymph nodes and protects against mucosal challenge [J].

Davis, NL ;

Brown, KW ;

Johnston, RE .

JOURNAL OF VIROLOGY, 1996, 70 (06) :3781-3787

[10] HUMAN ENTERIC CALICIVIRIDAE - THE COMPLETE GENOME SEQUENCE AND EXPRESSION OF VIRUS-LIKE PARTICLES FROM A GENETIC GROUP-II SMALL ROUND STRUCTURED VIRUS [J].

DINGLE, KE ;

LAMBDEN, PR ;

CAUL, EO ;

CLARKE, IN .

JOURNAL OF GENERAL VIROLOGY, 1995, 76 :2349-2355

← 1 2 3 4 5 →