Identification of ectomycorrhizae from Tuber species by RFLP analysis of the its region.

被引：28

作者：

Amicucci, A

Rossi, I

Potenza, L

Zambonelli, A

Agostini, D

Palma, F

Stocchi, V

机构：

[1] UNIV URBINO,IST CHIM BIOL GIORGIO FORNAINI,I-61029 URBINO,ITALY

[2] UNIV BOLOGNA,DIPARTIMENTO PROTEZ & VALORIZZAZIONE AGROALIMENTA,I-40126 BOLOGNA,ITALY

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 07期

关键词：

D O I：

10.1007/BF00127896

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polymorphisms of a ribosomal DNA I region (ITS) have been analysed using a specific pair of primers, in order Co type fruitbodies and ectomycorrhizae of different truffle species. The identification of ectomycorrhizae was obtained by digestion of the PCR products using restriction enzymes. The results show that the strategy used is both suitable and sensitive to characterize the symbiotic fungi from few mycorrhized root tips.

引用

页码：821 / 826

页数：6

共 20 条

[1]

[Anonymous], 1990, PCR protocols: a guide to methods and applications, DOI [10.1016/B978-0-12-372180-8.50038-X, DOI 10.1016/B978-0-12-372180-8.50038-X]

[2] IDENTIFICATION OF THE ECTOMYCORRHIZAL BASIDIOMYCETE TYLOSPORA-FIBRILLOSA DONK BY RFLP ANALYSIS OF THE PCR-AMPLIFIED ITS AND IGS REGIONS OF RIBOSOMAL DNA [J].