Collagenase degradation decreases collagen fibril diameters -: an in vitro study of the rabbit medial collateral ligament

被引:23
作者
Cunningham, KD
Musani, F
Hart, DA
Shrive, NG
Frank, CB
机构
[1] Univ Calgary, Fac Med, McCaig Ctr Joint Injury & Arthrit Res, Dept Med Sci, Calgary, AB T2N 4N1, Canada
[2] Univ Calgary, Fac Med, McCaig Ctr Joint Injury & Arthrit Res, Dept Microbiol & Infect Dis, Calgary, AB T2N 4N1, Canada
[3] Univ Calgary, Fac Med, McCaig Ctr Joint Injury & Arthrit Res, Dept Civil Engn, Calgary, AB T2N 4N1, Canada
[4] Univ Calgary, Fac Med, McCaig Ctr Joint Injury & Arthrit Res, Dept Surg,Div Orthopaed, Calgary, AB T2N 4N1, Canada
基金
英国医学研究理事会;
关键词
collagen; collagenase; medial collateral ligament; TEM;
D O I
10.3109/03008209909005279
中图分类号
Q2 [细胞生物学];
学科分类号
071009 [细胞生物学]; 090102 [作物遗传育种];
摘要
Based on the similarity of fibril diameters in healing and grafted ligaments, it has been speculated that all small fibrils represent newly synthesized collagen. Alternatively, small fibrils in grafts could be due to enzymatic degradation of endogenous large fibrils, This study examined the effect of collagenase on collagen fibril diameters in normal NZW rabbit MCLs, Midsubstance MCL slivers were incubated in buffer for 72 or 144 h for comparison with slivers incubated in buffer containing 4 units/ml bacterial collagenase. The samples were examined under TEM for fibril diameter analysis. Mean fibril diameters of 3-day and 6-day collagenase-treated MCLs were significantly reduced, resembling 40-week scar values. These results suggest that collagenase treatment can alter collagen fibril diameter and shape in normal rabbit MCL, thus it is possible that despite their similarity to ligament scars, that at least some small fibrils in ligament grafts mag be enzymatically reduced endogenous fibrils.
引用
收藏
页码:67 / 74
页数:8
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