Acute effect of fatty acids on metabolism and mitochondrial coupling in skeletal muscle

被引：64

作者：

Hirabara, SM

Silveira, LR

Alberici, LC

Leandro, CVG

Lambertucci, RH

Polimeno, GC

Boaventura, MFC

Procopio, J

Vercesi, AE

Curi, R

机构：

[1] Univ Sao Paulo, Cidade Univ, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo, Brazil

[2] Univ Estadual Campinas, Fac Med Sci, Dept Clin Pathol, Campinas, SP, Brazil

[3] Univ Pernambuco, Super Sch Phys Exercise, Recife, PE, Brazil

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2006年 / 1757卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

long-chain fatty acid; skeletal muscle; mitochondrial uncoupling; glucose metabolism; mitochondria;

D O I：

10.1016/j.bbabio.2005.11.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acute effects of free fatty acids (FFA) were investigated on: (1) glucose oxidation, and UCP-2 and -3 mRNA and protein levels in 1 h incubated rat soleus and extensor digitorium longus (EDL) muscles, (2) mitochondrial membrane potential in cultured skeletal muscle cells, (3) respiratory activity and transmembrane electrical potential in mitochondria isolated from rat skeletal muscle, and (4) oxygen consumption by anesthetized rats. Long-chain FFA increased both basal and insulin-stimulated glucose oxidation in incubated rat soleus and EDL muscles and reduced mitochondrial membrane potential in C2C12 myotubes and rat skeletal muscle cells. Caprylic, palmitic, oleic, and linoleic acid increased O-2 consumption and decreased electrical membrane potential in isolated mitochondria from rat skeletal muscles. FFA did not alter UCP-2 and -3 mRNA and protein levels in rat soleus and EDL muscles. Palmitic acid increased oxygen consumption by anesthetized rats. These results suggest that long-chain FFA acutely lead to mitochondrial uncoupling in skeletal muscle. (c) 2005 Elsevier B.V All rights reserved.

引用

页码：57 / 66

页数：10

共 58 条

[1] HINDLIMB MUSCLE FIBER POPULATIONS OF 5 MAMMALS [J].

ARIANO, MA ;

ARMSTRONG, RB ;

EDGERTON, VR .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1973, 21 (01) :51-55

[2] Rhodamine 123 as a probe of mitochondrial membrane potential:: evaluation of proton flux through F0 during ATP synthesis [J].

Baracca, A ;

Sgarbi, G ;

Solaini, G ;

Lenaz, G .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2003, 1606 (1-3) :137-146

[3] INTRACELLULAR SITE OF INSULIN ACTION - MITOCHONDRIAL KREBS CYCLE [J].

BESSMAN, SP ;

MOHAN, C ;

ZAIDISE, I .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (14) :5067-5070

[4] Constitutive UCP3 overexpression at physiological levels increases mouse skeletal muscle capacity for fatty acid transport and oxidation [J].

Bezaire, V ;

Spriet, LL ;

Campbell, S ;

Sabet, N ;

Gerrits, M ;

Bonen, A ;

Harper, ME .

FASEB JOURNAL, 2005, 19 (06) :977-+

[5] Uncoupling protein-3: A new member of the mitochondrial carrier family with tissue-specific expression [J].

Boss, O ;

Samec, S ;

PaoloniGiacobino, A ;

Rossier, C ;

Dulloo, A ;

Seydoux, J ;

Muzzin, P ;

Giacobino, JP .

FEBS LETTERS, 1997, 408 (01) :39-42

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[8] Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean [J].

Clapham, JC ;

Arch, JRS ;

Chapman, H ;

Haynes, A ;

Lister, C ;

Moore, GBT ;

Piercy, V ;

Carter, SA ;

Lehner, I ;

Smith, SA ;

Beeley, LJ ;

Godden, RJ ;

Herrity, N ;

Skehel, M ;

Changani, KK ;

Hockings, PD ;

Reid, DG ;

Squires, SM ;

Hatcher, J ;

Trail, B ;

Latcham, J ;

Rastan, S ;

Harper, AJ ;

Cadenas, S ;

Buckingham, JA ;

Brand, MD ;

Abuin, A .

NATURE, 2000, 406 (6794) :415-418

[9] INSULIN RESISTANCE IN SOLEUS MUSCLE FROM OBESE ZUCKER RATS - INVOLVEMENT OF SEVERAL DEFECTIVE SITES [J].

CRETTAZ, M ;

PRENTKI, M ;

ZANINETTI, D ;

JEANRENAUD, B .

BIOCHEMICAL JOURNAL, 1980, 186 (02) :525-534

[10] Selective detection of UCP 3 expression in skeletal muscle: effect of thyroid status and temperature acclimation [J].

Cunningham, O ;

McElligott, AM ;

Carroll, AM ;

Breen, E ;

Reguenga, C ;

Oliveira, MEM ;

Azevedo, JE ;

Porter, RK .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2003, 1604 (03) :170-179

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