Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression

被引：33

作者：

Lammers, Gerwen ^{[1
]}

Roth, Guenter ^{[2
,3
,4
]}

Heck, Mathias ^{[2
]}

Zengerle, Roland ^{[2
,3
,4
]}

Tjabringa, G. Sandra ^{[5
]}

Versteeg, Elly M. ^{[1
]}

Hafmans, Theo ^{[1
]}

Wismans, Ronnie ^{[1
]}

Reinhardt, Dieter P. ^{[6
]}

Verwiel, Eugene T. P. ^{[7
]}

Zeeuwen, Patrick L. J. M. ^{[5
]}

Schalkwijk, Joost ^{[5
]}

Brock, Roland ^{[1
]}

Daamen, Willeke F. ^{[1
]}

van Kuppevelt, Toin H. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Med Ctr, Dept Biochem 280, Nijmegen Ctr Mol Life Sci, NL-6500 HB Nijmegen, Netherlands

[2] Univ Freiburg, Dept Microsyst Engn IMTEK, Lab MEMS Applicat, D-79110 Freiburg, Germany

[3] HSG IMIT, D-78052 Villingen Schwenningen, Germany

[4] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, D-79110 Freiburg, Germany

[5] Radboud Univ Nijmegen, Med Ctr, Dept Dermatol 370, Nijmegen Ctr Mol Life Sci, NL-6500 HB Nijmegen, Netherlands

[6] McGill Univ, Dept Anat & Cell Biol, Montreal, PQ, Canada

[7] Radboud Univ Nijmegen, Med Ctr, Dept Human Genet 855, Nijmegen Ctr Mol Life Sci, NL-6500 HB Nijmegen, Netherlands

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 05期

关键词：

biomedical applications; biomimetics; microstructures; tissue engineering; STEM-CELLS; ATOPIC-DERMATITIS; HUMAN EPIDERMIS; HUMAN SKIN; PSORIASIS; DIFFERENTIATION; REGENERATION; PROGENY; ARREST; GROWTH;

D O I：

10.1002/mabi.201100443

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A papillary-structured collagen fibril membrane is created, mimicking the 3D-architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar-structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the presence of morphologically distinct cells at the base of the papillar structures that are not observed on flat membranes. Gene expression microarrays and RT-qPCR indicate that these cells are in a more proliferative/migrational state, whereas cells on flat membranes have a more differentiated expression profile. Immunohistochemical stainings confirm these results. In conclusion, specific collagen architecture can direct keratinocyte behavior, and this may be used to further improve skin regeneration.

引用

页码：675 / 691

页数：17

共 37 条

[1]

Psoriasis Risk Genes of the Late Cornified Envelope-3 Group Are Distinctly Expressed Compared with Genes of Other LCE Groups [J].