Immobilization of glutaryl-7-ACA acylase on aminoalkylated polyacrylic supports

被引：22

作者：

Bianchi, D

Golini, P

Bortolo, R

Battistel, E

Tassinari, R

Cesti, P

机构：

[1] Enichem S.p.A., Istituto G. Donegani, Novara

[2] Enichem S.p.A., Instituto Guido Donegani, 28100 Novara

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1997年 / 20卷 / 05期

关键词：

E-coli glutaryl-7-ACA acylase; covalent immobilization of enzymes; aminoalkylation of polyacrylester; glutaraldehyde coupling;

D O I：

10.1016/S0141-0229(96)00167-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A procedure is described for the immobilization of glutaryl-7-ACA acylase from recombinant E. coli on Amberlite XAD7 modified by transamidation with I,2-diaminoethane and activated with glutaraldehyde. The determination of the optimal operative conditions such as enzyme loading, effect of pH, temperature, and product inhibition is described for the hydrolysis of 7-beta-(4-carboxybutanamido) cephalosporanic acid (glutaryl-7-ACA) to give 7-aminocephalosporanic acid (7-ACA). The inactivation kinetics of the immobilized preparations determined in a continuous fixed-bed reactor are also reported. (C) 1997 by Elsevier Science Inc.

引用

页码：368 / 372

页数：5

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