In vitro propagation of Veltheimia bracteata and V-bracteata 'Lemon flame'

被引：5

作者：

Taylor, JLS ^{[1
]}

vanStaden, J ^{[1
]}

机构：

[1] UNIV NATAL PIETERMARITZBURG,DEPT BOT,RES UNIT PLANT GROWTH & DEV,ZA-3209 SCOTTSVILLE,SOUTH AFRICA

来源：

SOUTH AFRICAN JOURNAL OF BOTANY | 1997年 / 63卷 / 03期

关键词：

Veltheimia bracteata; micropropagation; tissue culture; conservation; horticulture;

D O I：

10.1016/S0254-6299(15)30729-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In vitro adventitious bud formation was initiated using leaf and bulb explants of both Veltheimia bracteata and V. bracteata 'Lemon Flame'. The initiation medium used consisted of Murashige and Skoog (MS) solidified with 0.8% agar, and was further supplemented with 100 mgl(-1) myo-inositol, 25 mgl(-1) NaFeEDTA, 0.5 mgl(-1) thiamine-HCl and 3% sucrose. Adventitious bud initiation was stimulated by a combination of BA (2 mgl(-1)) and NAA (0.1 mgl(-1)) Buds were transferred to MS medium supplemented with 100 mgl(-1) myo-inositol, 3% sucrose and IBA (2 mgl(-1)) for root initiation. Plantlets were successfully hardened off in vermiculite under misthouse conditions over a 4 week period. These plants were subsequently transferred to a greenhouse and watered twice weekly. Where was 98% survival of plantlets. Explants from the cultivar 'Lemon Flame', however produced 50% less plantlets in vitro.

引用

页码：159 / 161

页数：3