Fishing for ancient DNA

被引：38

作者：

Anderung, Cecilia ^{[1
]}

Persson, Per ^{[2
]}

Bouwman, Abigail ^{[3
]}

Elburg, Rengert ^{[4
]}

Goethertroem, Anders ^{[1
]}

机构：

[1] Uppsala Univ, Dept Evolut Genom & Systemat, Evolutionary Biol Ctr, SE-75236 Uppsala, Sweden

[2] Univ Oslo, Kulturhist Museum, N-0130 Oslo, Norway

[3] Univ Manchester, Manchester Interdisciplinary Bioctr, Fac Life Sci, Manchester M1 7DN, Lancs, England

[4] IO Graph Germany, D-01097 Dresden, Germany

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2008年 / 2卷 / 02期

关键词：

Ancient DNA extraction; Silica binding purification; Magnetic separation;

D O I：

10.1016/j.fsigen.2007.09.004

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The major problems concerning ancient DNA studies are related to the amount of extractable DNA and the precautions needed to avoid contamination. From the very first step of the analyses, the DNA extraction, these problems must be confronted. There are several extraction methods available for DNA in ancient tissue; several of them are complicated and time consuming. and none of the methods have reached an acceptance level such that they are routinely used on a widespread basis. Here we investigate the efficiency of two methods. one based on magnetic separation of the targeted molecules. and one based oil silica binding. The efficiency rate of these two on the material studied seems to be identical. The silica binding method has the benefit of relative simplicity, but the magnetic separation technique also hits advantages. For example, it is possible to reuse the extract several times for different loci, and it is possible to concentrate all extracted DNA from one locus into one PCR. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：104 / 107

页数：4

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