Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium

被引：38

作者：

Itaya, M ^{[1
]}

Sakaya, N ^{[1
]}

Matsunaga, S ^{[1
]}

Fujita, K ^{[1
]}

Kaneko, S ^{[1
]}

机构：

[1] Mitsubishi Kagaku Inst Life Sci, Machida, Tokyo 1948511, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 03期

关键词：

Bacillus subtilis; conjugational transfer; pLS20;

D O I：

10.1271/bbb.70.740

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient. Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.

引用

页码：740 / 742

页数：3

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