Increased miR-374b promotes cell proliferation and the production of aberrant glycosylated IgA1 in B cells of IgA nephropathy

被引：55

作者：

Hu, Shuai ^{[1
]}

Bao, Hao ^{[1
]}

Xu, Xiaodong ^{[1
]}

Zhou, Xianguang ^{[1
]}

Qin, Weisong ^{[1
]}

Zeng, Caihong ^{[1
]}

Liu, Zhihong ^{[1
]}

机构：

[1] Nanjing Univ, Sch Med, Jinling Hosp, Natl Clin Res Ctr Kidney Dis, Nanjing 210002, Jiangsu, Peoples R China

来源：

FEBS LETTERS | 2015年 / 589卷 / 24期

基金：

中国国家自然科学基金;

关键词：

MiR-374b; B cell; IgA nephropathy; O-GLYCOSYLATION; DOWN-REGULATION; CANCER CELLS; SERUM IGA1; COSMC; ACTIVATION; BETA-1,3-GALACTOSYLTRANSFERASE; EXPRESSION; PTEN;

D O I：

10.1016/j.febslet.2015.10.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The number of B cells is increased and the O-glycans of IgA1 are incompletely galactosylated in IgA nephropathy (IgAN). Here we report that expression of phosphatase and tensin homolog (PTEN) and Cosmc is decreased in B cells, and correlates with B cell number and the aberrant glycosylation of IgA1 in IgAN. Patients with IgAN exhibit higher miR-374b in B cells compared to controls. We show that miR-374b targets PTEN and Cosmc by luciferase assays and western blot analysis. Inhibition of miR-374b increased PTEN and Cosmc expression, and prevented cell proliferation and aberrant glycosylation of IgA1, thus representing a new therapeutic approach for IgAN. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4019 / 4025

页数：7

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