Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation

被引:300
作者
Cecchetti, Valentina [2 ]
Altamura, Maria Maddalena [3 ]
Falasca, Giuseppina [3 ]
Costantino, Paolo [2 ]
Cardarelli, Maura [1 ]
机构
[1] Univ Roma La Sapienza, CNR, Dipartimento Genet & Biol Mol, Ist Biol & Patol Mol, I-00185 Rome, Italy
[2] Univ Roma La Sapienza, Ist Pasteur Fdn Cenci Bolognetti, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy
[3] Univ Roma La Sapienza, Dipartimento Biol Vegetale, I-00185 Rome, Italy
关键词
D O I
10.1105/tpc.107.057570
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We provide evidence on the localization, synthesis, transport, and effects of auxin on the processes occurring late in Arabidopsis thaliana stamen development: anther dehiscence, pollen maturation, and preanthesis filament elongation. Expression of auxin-sensitive reporter constructs suggests that auxin effects begin in anthers between the end of meiosis and the bilocular stage in the somatic tissues involved in the first step of dehiscence as well as in the microspores and in the junction region between anther and filament. In situ hybridizations of the auxin biosynthetic genes YUC2 and YUC6 suggest that auxin is synthesized in anthers. In agreement with the timing of auxin effects, the TIR1, AFB1, AFB2, and AFB3 auxin receptor-encoding genes are transcribed in anthers only during late stages of development starting at the end of meiosis. We found that in tir1 afb triple and quadruple mutants, anther dehiscence and pollen maturation occur earlier than in the wild type, causing the release of mature pollen grains before the completion of filament elongation. We also assessed the contribution of auxin transport to late stamen developmental processes. Our results suggest that auxin synthesized in anthers plays a major role in coordinating anther dehiscence and pollen maturation, while auxin transport contributes to the independent regulation of preanthesis filament elongation.
引用
收藏
页码:1760 / 1774
页数:15
相关论文
共 35 条
[1]   Role of auxin in regulating Arabidopsis flower development [J].
Aloni, R ;
Aloni, E ;
Langhans, M ;
Ullrich, CI .
PLANTA, 2006, 223 (02) :315-328
[2]   The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots [J].
Blilou, I ;
Xu, J ;
Wildwater, M ;
Willemsen, V ;
Paponov, I ;
Friml, J ;
Heidstra, R ;
Aida, M ;
Palme, K ;
Scheres, B .
NATURE, 2005, 433 (7021) :39-44
[3]   ANTHER DEHISCENCE IN LYCOPERSICON-ESCULENTUM MILL [J].
BONNER, LJ ;
DICKINSON, HG .
NEW PHYTOLOGIST, 1989, 113 (01) :97-115
[4]  
Bowman J., 1994, ARABIDOPSIS ATLAS MO
[5]   Expression of rolB in tobacco flowers affects the coordinated processes of anther dehiscence and style elongation [J].
Cecchetti, V ;
Pomponi, M ;
Altamura, MM ;
Pezzotti, M ;
Marsilio, S ;
D'Angeli, S ;
Tornielli, GB ;
Costantino, P ;
Cardarelli, M .
PLANT JOURNAL, 2004, 38 (03) :512-525
[6]   ROX1, a gene induced by rolB, is involved in procambial cell proliferation and xylem differentiation in tobacco stamen [J].
Cecchetti, Valentina ;
Altamura, Maria Maddalena ;
Serino, Giovanna ;
Pomponi, Mirella ;
Falasca, Giuseppina ;
Costantino, Paolo ;
Cardarelli, Maura .
PLANT JOURNAL, 2007, 49 (01) :27-37
[7]   Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis [J].
Cheng, Youfa ;
Dai, Xinhua ;
Zhao, Yunde .
GENES & DEVELOPMENT, 2006, 20 (13) :1790-1799
[8]   The auxin-binding protein 1 is essential for the control of cell cycle [J].
David, Karine M. ;
Couch, Daniel ;
Braun, Nils ;
Brown, Spencer ;
Grosclaude, Jeanne ;
Perrot-Rechenmann, Catherine .
PLANT JOURNAL, 2007, 50 (02) :197-206
[9]   Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium [J].
Dawson, J ;
Sözen, E ;
Vizir, I ;
Van Waeyenberge, S ;
Wilson, ZA ;
Mulligan, BJ .
NEW PHYTOLOGIST, 1999, 144 (02) :213-222
[10]   The plant cell cycle [J].
Dewitte, W ;
Murray, JAH .
ANNUAL REVIEW OF PLANT BIOLOGY, 2003, 54 :235-264