Effects of tartrazine on exploratory behavior in a three-generation toxicity study in mice

Tartrazine was given to mice in the diet at levels of 0 (control), 0.05%,0.15%, and 0.45% from 5 weeks of age of the F-0 generation to 9 weeks of age of the F-2 generation, and selected reproductive and neurobehavioral parameters were measured. In the F, generation, the development of swimming direction at postnatal day (PND) 7 was accelerated significantly in male offspring in a dose-related manner. Surface righting at PND 7 was affected significantly in female offspring in dose-related manner. Several variables in exploratory behavior showed significant tendencies to be affected in the treatment groups in male offspring at 3 weeks ofage. In the F2 generation, the development of swimming direction at PND 7 was accelerated significantly in the high-dosed group in male offspring. Time taken of olfactory orientation at PND 14 was accelerated significantly in male offspring in a dose-related manner. Several variables in exploratory behavior showed significant tendencies to be affected in the treatment groups in male offspring at 3 weeks of age, and in males at 8 weeks ofage. The dose levels of tartrazine in the present study produced a few adverse effects on neurobehavioral parameters throughout generations in mice. (C) 2008 Elsevier Inc. All rights reserved.