FTIR-determination of sterols from the red alga Asparagopsis armata:: Comparative studies with HPLC

被引：17

作者：

El Hattab, N

Daghbouche, Y

El Hattab, M

Piovetti, L

Garrigues, S

de la Guardia, M

机构：

[1] Univ Blida, Dept Chem, Lab Mol & Macromol Phys Chem, Blida 09000, Algeria

[2] Univ S Toulon Var, Lab MFS, Team Marine Nat Prod, F-83957 La Garde, France

[3] Univ Valencia, Dept Analyt Chem, E-46003 Valencia, Spain

来源：

TALANTA | 2006年 / 68卷 / 04期

关键词：

Fourier transform infrared; solid phase extraction; high performance liquid chromatography; sterol determination; red alga; Asparagopsis armata;

D O I：

10.1016/j.talanta.2005.07.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An analytical procedure was developed for the determination of the total amount of sterols in the red alga Asparagopsis armata, globally determined as cholesterol, which is the major sterol contained in red algae. Samples, previously saponified with KOH were preconcentrated on DSC-18 solid phase cartridges (SPE) and eluted with dichloromethane stabilized with beta-amylene. Fourier transform infrared (FTIR) spectrometry was employed for selective detection at 1049 cm(-1) with a baseline established between 1000 and 1079 cm(-1). The results were compared to those obtained by high performance liquid chromatography (HPLC). The concentration obtained in actual samples from alga was 3.37% (w/w) by FTIR and 3.30% (w/w) by HPLC, showing a good comparability between the two methods. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：1230 / 1235

页数：6

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