Serine-threonine protein kinase activity of Elm1p, a regulator of morphologic differentiation in Saccharomyces cerevisiae

被引：20

作者：

Koehler, CM ^{[1
]}

Myers, AM ^{[1
]}

机构：

[1] IOWA STATE UNIV, DEPT BIOCHEM & BIOPHYS, AMES, IA 50011 USA

来源：

FEBS LETTERS | 1997年 / 408卷 / 01期

基金：

美国国家科学基金会;

关键词：

serine-threonine protein kinase; (S-cerevisiae); pseudohyphal growth; serine-threonine autophosphorylation; baculovirus expression system; kinase-negative mutant;

D O I：

10.1016/S0014-5793(97)00401-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Saccharomyces cerevisiae gene ELM1 regulates morphologic differentiation and its nucleotide sequence predicts a novel protein kinase. Elm1p was expressed in yeast and insect cells and purified. Elm1p displayed protein kinase activity in autophosphorylation assays and towards exogenous substrates. Serine and threonine residues were identified as the accepters in these reactions. These data together with previous genetic analysis of ELM1 function indicate that phosphorylation on serine and/or threonine residues of a particular substrate or set of substrates by Elm1p is required for repression of the filamentous growth differentiation state. (C) 1997 Federation of European Biochemical Societies.

引用

页码：109 / 114

页数：6

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