Functional expression of the tandem-repeated homodimer of the mitochondrial ADP/ATP carrier in Saccharomyces cerevisiae

被引：39

作者：

Hatanaka, T

Hashimoto, M

Majima, E

Shinohara, Y

Terada, H

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan

[2] APRO Life Sci Inst, Naruto 7720001, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 262卷 / 03期

关键词：

D O I：

10.1006/bbrc.1999.1283

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitochondrial ADP/ATP carrier (AAC) is believed to function as a dimer. To characterize the oligomeric state of the yeast type 2 AAC (yAAC2), we tried to express its tandem-repeated homodimer, in which the C-terminus of the first repeat was fused to the N-terminus of the second repeat, in yeast mitochondria. The tandem dimer was expressed in the mitochondrial membrane at the same level as that of yAAC2, being inserted into the mitochondrial membrane as in yAAC2, and it showed very similar transport activity to that of yAAC2. It was suggested that the two carrier molecules in a dimeric form are located in the membrane facing each other in the same orientation. (C) 1999 Academic Press.

引用

收藏

页码：726 / 730

页数：5

相关论文

共 20 条

[1] SEQUENCES REQUIRED FOR DELIVERY AND LOCALIZATION OF THE ADP/ATP TRANSLOCATOR TO THE MITOCHONDRIAL INNER MEMBRANE [J].

ADRIAN, GS ;

MCCAMMON, MT ;

MONTGOMERY, DL ;

DOUGLAS, MG .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (02) :626-634

[2] ORIENTATION OF THE N-TERMINAL REGION OF THE MEMBRANE-BOUND ADP ATP CARRIER PROTEIN EXPLORED BY ANTIPEPTIDE ANTIBODIES AND AN ARGININE-SPECIFIC ENDOPROTEASE - EVIDENCE THAT THE ACCESSIBILITY OF THE N-TERMINAL RESIDUES DEPENDS ON THE CONFORMATIONAL STATE OF THE CARRIER [J].

BRANDOLIN, G ;

BOULAY, F ;

DALBON, P ;

VIGNAIS, PV .

BIOCHEMISTRY, 1989, 28 (03) :1093-1100

[3] MOLECULAR-WEIGHT AND HYDRODYNAMIC PARAMETERS OF THE ADENOSINE 5'-DIPHOSPHATE-ADENOSINE 5'-TRIPHOSPHATE CARRIER IN TRITON X-100 [J].

HACKENBERG, H ;

KLINGENBERG, M .

BIOCHEMISTRY, 1980, 19 (03) :548-555

[4] Expression of the bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria: significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carriers [J].

Hashimoto, M ;

Shinohara, Y ;

Majima, E ;

Hatanaka, T ;

Yamazaki, N ;

Terada, H .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1999, 1409 (03) :113-124

[5] Fluctuation of the first loop facing the matrix of the mitochondrial ADP/ATP carrier deduced from intermolecular cross-linking of Cys56 residues by bifunctional dimaleimides [J].

Hashimoto, M ;

Majima, E ;

Goto, S ;

Shinohara, Y ;

Terada, H .

BIOCHEMISTRY, 1999, 38 (03) :1050-1056

[6]

HUANG SS, IN PRESS

[7] THE ISOLATION AND RECONSTITUTION OF THE ADP ATP CARRIER FROM WILD-TYPE SACCHAROMYCES-CEREVISIAE - IDENTIFICATION OF PRIMARILY ONE TYPE (AAC-2) [J].

KNIRSCH, M ;

GAWAZ, MP ;

KLINGENBERG, M .

FEBS LETTERS, 1989, 244 (02) :427-432

[8]

KOLAROV J, 1990, J BIOL CHEM, V265, P12711

[9] RECONSTITUTION OF ADENINE-NUCLEOTIDE TRANSPORT WITH PURIFIED ADP, ATP-CARRIER PROTEIN [J].

KRAMER, R ;

KLINGENBERG, M .

FEBS LETTERS, 1977, 82 (02) :363-367

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+