Induction of ischemic tolerance in rat cortical neurons by 3-nitropropionic acid: chemical preconditioning

被引：35

作者：

Weih, M

Bergk, A

Isaev, NK

Ruscher, K

Megow, D

Riepe, M

Meisel, A

Victorov, IV

Dirnagi, U

机构：

[1] Charite, Dept Neurol, Berlin, Germany

[2] Russian Acad Med Sci, Brain Res Inst, Moscow, Russia

[3] Univ Ulm, Dept Neurol, D-7900 Ulm, Germany

来源：

NEUROSCIENCE LETTERS | 1999年 / 272卷 / 03期

关键词：

cerebral ischemia; hypoxia; cell culture; transcription factors; stroke; oxidative phosphorylation;

D O I：

10.1016/S0304-3940(99)00594-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Sublethal ischemia leads to increased tolerance against subsequent ischemia. We investigated whether tolerance could also be elicited by mild respiratory-chain inhibition (chemical hypoxia) in a rat neuronal-cell enriched culture system. 3-Nitropropionic acid (3-NPA) caused a concentration-dependent inhibition of succinate-dehydrogenase. Two hours preconditioning with 3-NPA 24-48 h before oxygen-glucose deprivation (OGD) reduced neuronal damage morphologically and reduced lactate deydrogenase (LDH) release up to 72% compared to sham-treated sister cultures without 3-NPA. In an attempt to elucidate transcriptional mechanisms, we found no rapid translocation of the hypoxia-sensitive transcription factors N F-KB or hypoxia-inducible factor-1 (HIF-1) at 3-NPA concentrations sufficient to trigger tolerance against OGD. In accordance to previous in vivo and brain slice data, we conclude that 8-NPA chemically induces tolerance against oxygen-glucose deprivation in vitro. However, the underlying mechanisms remain elusive. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：207 / 210

页数：4

共 20 条

[1] Ischemic preconditioning and brain tolerance - Temporal histological and functional outcomes, protein synthesis requirement, and interleukin-1 receptor antagonist and early gene expression [J].