How to prove the existence of metabolons?

被引：29

作者：

Bassard, Jean-Etienne ^{[1
]}

Halkier, Barbara Ann ^{[2
]}

机构：

[1] Univ Copenhagen, Plant Biochem Lab, Ctr Synthet Biol, VILLUM Res Ctr Plant Plast,Dept Plant & Environm, Copenhagen, Denmark

[2] Univ Copenhagen, DynaMo Ctr, Dept Plant & Environm Sci, Copenhagen, Denmark

来源：

PHYTOCHEMISTRY REVIEWS | 2018年 / 17卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Fluorescence-based protein-protein interaction; Fluorescence correlation spectroscopy; Fluorescence lifetime imaging microscopy; Yeast-2-hybrid screen; PROTEIN-PROTEIN INTERACTIONS; LIFETIME IMAGING MICROSCOPY; BIMOLECULAR FLUORESCENCE COMPLEMENTATION; TANDEM AFFINITY PURIFICATION; ENDOPLASMIC-RETICULUM; MEMBRANE-PROTEINS; MASS-SPECTROMETRY; SPLIT-UBIQUITIN; LIVING CELLS; PLANT-CELLS;

D O I：

10.1007/s11101-017-9509-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Sequential enzymes in biosynthetic pathways are organized in metabolons. It is challenging to provide experimental evidence for the existence of metabolons as biosynthetic pathways are composed of highly dynamic protein-protein interactions. Many different methods are being applied, each with strengths and weaknesses. We will present and evaluate several techniques that have been applied in providing evidence for the orchestration of the biosynthetic pathways of cyanogenic glucosides and glucosinolates in metabolons. These evolutionarily related pathways have ER-localized cytochromes P450 that are proposed to function as anchoring site for assembly of the enzymes into metabolons. Additionally, we have included commonly used techniques, even though they have not been used (yet) on these two pathways. In the review, special attention will be given to less-exploited fluorescence-based methods such as FCS and FLIM. Ultimately, understanding the orchestration of biosynthetic pathways may contribute to successful engineering in heterologous hosts.

引用

页码：211 / 227

页数：17

共 103 条

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