Characterization and antioxidant activity of a polysaccharide extracted from Sarcandra glabra

被引：103

作者：

Jin, Lei ^{[1
]}

Guan, Xin ^{[1
]}

Liu, Wei ^{[1
]}

Zhang, Xian ^{[1
]}

Yan, Wei ^{[1
]}

Yao, Wenbing ^{[1
]}

Gao, Xiangdong ^{[1
]}

机构：

[1] China Pharmaceut Univ, Sch Life Sci & Technol, State Key Lab Nat Med, Nanjing 210009, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2012年 / 90卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Sarcandra glabra polysaccharide; Box-Behnken design; Monosaccharide composition; Antioxidant activity; LIPID-PEROXIDATION; PURIFICATION;

D O I：

10.1016/j.carbpol.2012.05.074

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Response surface methodology (RSM) was employed to optimize the parameters for polysaccharide extracted from the aerial parts of Sarcandra glabra (SGP). The optimum conditions were predicted as follows, ratio of water to raw material at 30, extracting temperature at 85 degrees C. extracting duration at 3 h, and yield was estimated at 4.55%. The experimental yield of SGP under the optimum conditions was 4.49 +/- 0.09%. A homogenous polysaccharide (SGP-1) was obtained by purification using DEAE-cellulose-52 and Sephacryl S-400 column chromatography. SGP-1 showed a single symmetrical peak in high performance size-exclusion chromatography (HPSEC) and the average molecular weight (Mw) was estimated to be 1.06 x 10(4) Da. It was composed of glucose, galactose, and mannose in a ratio of 8.38:3.13:1 determined by gas chromatography (GC). The in vitro antioxidant tests showed that SGP-1 has significant inhibition effects on hydroxyl, superoxide anion, DPPH. ABTS radicals in a dose-dependent manner. This study indicated that SGP-1 could be used as a potential natural antioxidant. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：524 / 532

页数：9

共 29 条

[1]

In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (Marine Brown Alga) [J].