Role of catalase in the smooth muscle relaxant actions of sodium azide and cyanamide

被引:17
作者
Shahidullah, M [1 ]
Duncan, A [1 ]
Strachan, PD [1 ]
Rafique, KM [1 ]
Ball, SL [1 ]
McPate, MJW [1 ]
Nelli, S [1 ]
Martin, W [1 ]
机构
[1] Univ Glasgow, Inst Biomed & Life Sci, Div Neurosci & Biomed Syst, Glasgow G12 8QQ, Lanark, Scotland
基金
英国惠康基金;
关键词
retractor penis muscle; bovine; catalase; nitric oxide (NO); nitroxyl anion; nitrovasodilator; aorta; rat;
D O I
10.1016/S0014-2999(01)01540-0
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The aim of this study was to determine the role of catalase in the smooth muscle relaxant actions of sodium azide and cyanamide. The effects of 3-amino-1,2,4-triazole suggested a role for this enzyme in the relaxant actions of sodium azide on rat aorta and bovine retractor penis muscle and cyanamide on rat aorta. Moreover, results obtained using a difference spectrophotometric assay based upon the oxidation of haemoglobin were consistent with the catalase-dependent oxidation of sodium azide to nitric oxide (NO) and of cyanamide to nitroxyl anion. Surprisingly, however, no free nitric oxide or nitroxyl was detected in solution using a sensitive electrode. This anomaly might be explained if the stable complexes of catalase with nitric oxide or nitroxyl do not release their respective ligand except to sites of high affinity, such as the haemoglobin employed in the difference spectrophotometric assay, or indeed, the soluble guanylate cyclase within the smooth muscle. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:93 / 101
页数:9
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