Regulated and polarized Ptdlns(3,4,5)P3 accumulation is essential for apical membrane morphogenesis in photoreceptor epithelial cells

被引:130
作者
Pinal, N
Goberdhan, DCI
Collinson, L
Fujita, Y
Cox, IM
Wilson, C
Pichaud, F
机构
[1] UCL, MRC, Mol Cell Biol Lab, London WC1E 6BT, England
[2] UCL, Cell Biol Unit, Dept Anat & Dev Biol, London WC1E 6BT, England
[3] UCL, Dept Biol, London WC1E 6BT, England
[4] Univ Oxford, Dept Human Anat & Genet, Oxford OX1 3QX, England
基金
英国医学研究理事会;
关键词
D O I
10.1016/j.cub.2005.11.068
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: In a specialized epithelial cell such as the Drosophila photoreceptor, a conserved set of proteins is essential for the establishment of polarity, its maintenance, or both-in Drosophila, these proteins include the apical factors Bazooka, D-atypical protein kinase C, and D-Par6 together with D-Ecadherin. However, little is known about the mechanisms by which such apical factors might regulate the differentiation of the apical membrane into functional domains such as an apical-most stack of microvilli or more lateral sub-apical membrane. Results: We show that in photoreceptors; Bazooka (D-Par3) recruits the tumor suppressor lipid phosphatase PTEN to developing cell-cell junctions (Zonula Adherens, za). za-localized PTEN controls the spatially restricted accumulation of optimum levels of the lipid PtdIns(3,4,5)P3 within the apical membrane domain. This in turn finely tunes activation of Akt1, a process essential for proper morphogenesis of the light-gathering organelle, consisting of a stack of F-actin rich microvilli within the apical membrane. Conclusions: Spatially localized PtdIns(3,4,5)P3 mediates directional sensing during neutrophil and Dictyostelium chemotaxis. We conclude that a conserved mechanism also operates during photoreceptor epithelial cell morphogenesis in order to achieve normal differentiation of the apical membrane.
引用
收藏
页码:140 / 149
页数:10
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