The regulation of toll-like receptor 2 by miR-143 suppresses the invasion and migration of a subset of human colorectal carcinoma cells

被引:96
作者
Guo, Haiyan [1 ]
Chen, Ying [2 ,3 ]
Hu, Xiaobo [1 ]
Qian, Guanxiang [4 ]
Ge, Shengfang [3 ,4 ]
Zhang, Jianjun [2 ]
机构
[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 3, Sch Med, Dept Clin Lab, Shanghai 200019, Peoples R China
[2] Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Dept Oral & Maxillofacial Head Neck Oncol, Shanghai 200011, Peoples R China
[3] Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Dept Ophthalmol, Shanghai 200030, Peoples R China
[4] Shanghai Jiao Tong Univ, Sch Med, Dept Biochem & Mol Biol, Shanghai 200030, Peoples R China
基金
中国国家自然科学基金;
关键词
miR-143; Toll-like receptor 2; Colorectal carcinoma; Invasion; Migration; INCREASED EXPRESSION; IMMUNE-SYSTEM; TUMOR; METASTASIS; RECOGNITION; MECHANISMS; GROWTH; INFLAMMATION; STIMULATION; DISEASE;
D O I
10.1186/1476-4598-12-77
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Background: The Toll-like receptor 2 (TLR2)-driven tissue response may promote neoangiogenesis and tumour growth by mechanisms that are poorly understood. Methods: We investigated the expression levels of TLR2 and associated-miRNAs in colorectal carcinoma (CRC) tissues and cell lines using real-time PCR, northern blotting and western blotting. Survival curver was generated by Log-Rank test and the role of TLR2 signalling in tumour invasion and migration was determined by transwell analysis kits. Results: We observed that the tissues from CRC patients express relatively high levels of TLR2. Targeting TLR2 markedly reduces the invasion and migration of CRC cells. We also found that miR-143, a putative tumour suppressor that is down-regulated in CRC tissues, reduces the invasion and migration of CRC cells primarily via TLR2. Utilising a xenograft mouse model, we demonstrated that re-expression of miR-143 inhibits CRC cell colonisation in vivo. Conclusion: miR-143 blocks the TLR2 signalling pathway in human CRC cells. This knowledge may pave the way for new clinical applications utilising miR-143 mimics in the treatment of patients with CRC.
引用
收藏
页数:10
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