Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients

被引：38

作者：

Nieto-Fernandez, FE ^{[1
]}

Mattocks, D

Cavani, F

Salzet, M

Stefano, GB

机构：

[1] SUNY Coll Old Westbury, Neurosci Res Inst, Old Westbury, NY 11568 USA

[2] Univ Sci & Tech Lille Flandres Artois, Lab Biol Anim SN3, F-59655 Villeneuve Dascq, France

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1999年 / 123卷 / 03期

关键词：

calcium transients; immunocytes; invertebrates; morphine; opioid; opiate; mu(3)-receptor; nitric oxide; naloxone; cNOS;

D O I：

10.1016/S0305-0491(99)00074-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Morphine significantly stimulated invertebrate immunocyte intracellular calcium level increases in a concentration-dependent manner in cells preloaded with Fura(TM) 2/AM. Morphine's action was blocked by prior exposure of the cells to the opiate receptor antagonist naloxone. Various opioid peptides did not exhibit this ability, indicating a morphine-mu(3) mediated process. In comparing the sequence of events concerning morphine's action in stimulating both [Ca2+](i) and NO production in these cells, we found that the first event precedes the second by 42 +/- 7 s. The opiate stimulation of [Ca2+](i) was attenuated in cells leached of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity in invertebrate immunocytes. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：295 / 299

页数：5

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