1',5'-anhydrohexitol oligonucleotides: Hybridisation and strand displacement with oligoribonucleotides, interaction with RNase H and HIV reverse transcriptase

被引：79

作者：

Hendrix, C ^{[1
]}

Rosemeyer, H ^{[1
]}

DeBouvere, B ^{[1
]}

VanAerschot, A ^{[1
]}

Seela, F ^{[1
]}

Herdewijn, P ^{[1
]}

机构：

[1] UNIV OSNABRUCK, INST CHEM, ORGAN & BIOORGAN CHEM LAB, D-49069 OSNABRUCK, GERMANY

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 1997年 / 3卷 / 09期

关键词：

duplex; gel mobility; nucleic acids; oligonucleotides; strand displacement;

D O I：

10.1002/chem.19970030920

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Hexitol nucleic acids (HNAs) with four natural bases form stable and sequence-selective duplexes with RNA. This was investigated by T-m determinations and gel shift experiments. The CD spectra of an HNA-RNA duplex show similarities with the CD spectra of the A-form of dsRNA. Single-stranded HNAs are able to induce strand displacement in a double-stranded RNA sequence. An HNA-RNA duplex is a poor substrate for RNase H, and can inhibit the RNase H-mediated cleavage of a natural DNA-RNA substrate. The HNA-RNA hybrid enhances the activity of HIV reverse transcriptase.

引用

页码：1513 / 1520

页数：8

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