Resveratrol Protects Mouse Oocytes from Methylglyoxal-Induced Oxidative Damage

被引：60

作者：

Liu, Yu ^{[1
]}

He, Xiao-Qin ^{[2
]}

Huang, Xin ^{[1
]}

Ding, Lu ^{[3
]}

Xu, Lin ^{[1
]}

Shen, Yu-Ting ^{[2
]}

Zhang, Fei ^{[2
]}

Zhu, Mao-Bi ^{[1
]}

Xu, Bai-Hui ^{[1
]}

Qi, Zhong-Quan ^{[1
]}

Wang, Hai-Long ^{[1
]}

机构：

[1] Xiamen Univ, Coll Med, Organ Transplantat Inst, Xiamen, Fujian Province, Peoples R China

[2] Xiamen Univ, Dept Obstet & Gynaecol, Zhongshan Hosp, Xiamen, Fujian Province, Peoples R China

[3] Xiamen Matern & Child Hlth Care Hosp, Ctr Reprod Med, Xiamen, Fujian Province, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 10期

基金：

中国国家自然科学基金;

关键词：

DNA-DAMAGE; PARTHENOGENETIC ACTIVATION; CHROMOSOME SEGREGATION; MAMMALIAN OOCYTES; INDUCED APOPTOSIS; CARBONYL STRESS; LDL OXIDATION; IN-VITRO; ANTIOXIDANT; CHECKPOINT;

D O I：

10.1371/journal.pone.0077960

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Methylglyoxal, a reactive dicarbonyl compound, is mainly formed from glycolysis. Methylglyoxal can lead to the dysfunction of mitochondria, the depletion of cellular anti-oxidation enzymes and the formation of advanced glycation ends. Previous studies showed that the accumulation of methylglyoxal and advanced glycation ends can impair the oocyte maturation and reduce the oocyte quality in aged and diabetic females. In this study, we showed that resveratrol, a kind of phytoalexin found in the skin of grapes, red wine and other botanical extracts, can alleviate the adverse effects caused by methylglyoxal, such as inhibition of oocyte maturation and disruption of spindle assembly. Besides, methylglyoxal-treated oocytes displayed more DNA double strands breaks and this can also be decreased by treatment of resveratrol. Further investigation of these processes revealed that methylglyoxal may affect the oocyte quality by resulting in excessive reactive oxygen species production, aberrant mitochondrial distribution and high level lipid peroxidation, and resveratrol can block these cytotoxic changes. Collectively, our results showed that resveratrol can protect the oocytes from methylglyoxal-induced cytotoxicity and this was mainly through the correction of the abnormity of cellular reactive oxygen species metabolism.

引用

页数：8

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