Modified pulsed-field gel electrophoresis method for DNA degradation-sensitive Salmonella enterica and Escherichia coli strains

被引：45

作者：

Liesegang, A ^{[1
]}

Tschäpe, H ^{[1
]}

机构：

[1] Robert Koch Inst, Natl Reference Ctr Salmonella & Other Enter, D-38855 Wernigerode, Germany

来源：

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY | 2002年 / 291卷 / 08期

关键词：

PFGE pattern analysis; DNA degradation; Tris radicals; thiourea;

D O I：

10.1078/1438-4221-00180

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

A number of S. enterica and E. coli strains appeared sensitive to a rapid DNA degradation during the course of PFGE pattern analysis. This kind of DNA degradation could not be stopped by intensive treatment with proteinase K, formalin treatment, or other modifications of the protocol for the isolation of intact chromosomal DNA. However, the application of 100 muM thiourea into the running buffer gave rise to clear-cut PFGE patterns and in turn to an overall typeability.

引用

页码：645 / 648

页数：4

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