Releasable luciferin-transporter conjugates: Tools for the real-time analysis of cellular uptake and release

被引：116

作者：

Jones, Lisa R.

Goun, Elena A.

Shinde, Rajesh

Rothbard, Jonathan B.

Contag, Christopher H.

Wender, Paul A. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Mol Pharmacol, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Pediat, Stanford, CA 94305 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 20期

关键词：

D O I：

10.1021/ja0586283

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The design, synthesis, and evaluation of conjugates of arginine-rich transporters and luciferin are described that release luciferin only after entry into cells that are stably transfected with luciferase. Each molecule of free luciferin that is released after entry generates a photon that can be measured allowing for real-time quantification of uptake and release in cells. The process provides a method to assay uptake and release of free luciferin as a function of variations in the releasable linker and in the transporter. Copyright © 2006 American Chemical Society.

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收藏

页码：6526 / 6527

页数：2

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