Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1

被引：35

作者：

Akiyama, K

Fujita, T

Kuroshima, K

Sakane, T

Yokota, A

Takata, R

机构：

[1] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan

[2] Takeda Chem Ind Ltd, Div Pharmaceut Res, Yodogawa Ku, Osaka 5328686, Japan

[3] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 87卷 / 03期

关键词：

Bacillus ehimensis; chitosanase; purification; cloning;

D O I：

10.1016/S1389-1723(99)80050-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus ehimensis EAG1 (IFO15659) produced and secreted chitosanase in the presence of exogenous chitosan. The chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approximately 31,000, A 1.9-kbp DNA fragment containing the chitosanase gene was cloned and the complete nucleotide sequence was determined. The sequence was found to contain a single open reading frame encoding a protein of 302 amino acids. The deduced amino acid sequence showed significant homology with the chitosanase from Bacillus circulans MH-K1.

引用

页码：383 / 385

页数：3

共 22 条

[1]

Akiyama Kouichi, 1997, Annals of the Phytopathological Society of Japan, V63, P385, DOI 10.3186/jjphytopath.63.385

[2] FUNGICIDAL EFFECT OF CHITOSAN ON FUNGI OF VARYING CELL-WALL COMPOSITION [J].